Department of Microbiology, Nippon Dental University School of Life Dentistry at Tokyo, Tokyo, Japan.
J Oral Biosci. 2020 Dec;62(4):315-321. doi: 10.1016/j.job.2020.09.004. Epub 2020 Sep 13.
Porphyromonas gingivalis is one of the etiologic agents of chronic periodontitis. Our previous study showed that the use of minimal media for P. gingivalis allowed to isolate novel inhibitors of P. gingivalis growth. However, growth of P. gingivalis in minimal media was not always reproducible.
To explain this phenomenon, we analyzed the growth of seven wild-type ATCC 33277 strains and two wild-type W83 strains in 10 minimal media and three complex media.
All nine strains grew in LF (Lactalbumin-Ferric chloride), GC (bovine γ-immunoglobulin G-Calcium chloride), and newly developed mC (milk-Casein) minimal media. Therefore, LF, GC, and mC could be used as minimal media for P. gingivalis. In contrast, other six minimal media containing bovine serum albumin (BSA) supported the growth of several less strains; among these, two media also showed lack of reproducibility in growth among ATCC 33277 strains. On the other hand, four ATCC 33277 strains grew similarly in all 13 media, but two W83 and other three ATCC 33277 strains grew differently in at least one medium.
These results suggest that the lack of reproducibility of P. gingivalis growth on minimal media is caused by the presence of BSA, and by differences among the standard strains of P. gingivalis.
牙龈卟啉单胞菌是慢性牙周炎的病因之一。我们之前的研究表明，使用最小培养基可以分离到牙龈卟啉单胞菌生长的新型抑制剂。然而，牙龈卟啉单胞菌在最小培养基中的生长并不总是具有重现性。
为了解释这一现象，我们分析了七种野生型 ATCC 33277 株和两种野生型 W83 株在 10 种最小培养基和三种复杂培养基中的生长情况。
所有 9 株菌均在 LF（乳白蛋白-三价铁）、GC（牛γ-免疫球蛋白 G-氯化钙）和新开发的 mC（牛奶-酪蛋白）最小培养基中生长。因此，LF、GC 和 mC 可作为牙龈卟啉单胞菌的最小培养基。相比之下，其他六种含有牛血清白蛋白（BSA）的最小培养基仅支持少数几种菌株的生长；其中，两种培养基在 ATCC 33277 株之间的生长重现性也较差。另一方面，四个 ATCC 33277 株在所有 13 种培养基中的生长情况相似，但两个 W83 和其他三个 ATCC 33277 株在至少一种培养基中的生长情况不同。
这些结果表明，最小培养基中牙龈卟啉单胞菌生长的重现性差是由 BSA 的存在以及牙龈卟啉单胞菌标准株之间的差异引起的。
