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迷迭香酸通过调节NF-κβ信号通路保护β细胞免受链脲佐菌素诱导的细胞损伤。

Rosemarinic acid protects β-cell from STZ-induced cell damage via modulating NF-κβ pathway.

作者信息

El-Huneidi Waseem, Anjum Shabana, Mohammed Abdul Khader, Bin Eshaq Shuhd, Abdrabh Sham, Bustanji Yasser, Soares Nelson C, Semreen Mohammad H, Alzoubi Karem H, Abu-Gharbieh Eman, Taneera Jalal

机构信息

Research Institute of Medical and Health Sciences, University of Sharjah, Sharjah, P.O. Box 27272, United Arab Emirates.

College of Medicine, University of Sharjah, Sharjah, P.O. Box 27272, United Arab Emirates.

出版信息

Heliyon. 2023 Aug 23;9(9):e19234. doi: 10.1016/j.heliyon.2023.e19234. eCollection 2023 Sep.

Abstract

Rosmarinic acid (RA), a natural ester phenolic compound, is known to have antioxidant and anti-inflammatory properties. RA has also been reported to exhibit a hypoglycemic effect; however, the mechanisms underlying this effect have yet to be investigated. Therefore, the present study focused on the anti-diabetic effects and mechanism of RA in INS-1 cells using model. Streptozotocin (STZ) at a concentration of 3 mM was applied to INS-1 cells for 4 h to create a diabetic model. The cells were pretreated for 24 h with various concentrations (1 and 2.5 μM) of RA. The Cell viability, glucose-stimulated insulin secretion (GSIS), glucose uptake, lipid peroxidation, reactive oxygen species (ROS), apoptosis, and protein expression of Bcl-2, NF-κB, 1L-1β, and PARP were assessed. Results showed that STZ-treated INS-1 cells exhibited reduced cell viability, insulin release, insulin content, glucose uptake, and elevated MDA and ROS levels. Cells pretreated with RA maintained the function and morphology of β-cells against STZ-induced damage. Moreover, RA sustained high protein expression levels of Bcl-2 and low expression levels of NF-κB, IL-1β, and PARP. In conclusion, RA preserved β-cells function against STZ-induced damage by altering NF-κB and Bcl-2 pathways.

摘要

迷迭香酸(RA)是一种天然的酯类酚类化合物,已知具有抗氧化和抗炎特性。也有报道称RA具有降血糖作用;然而,这种作用的潜在机制尚未得到研究。因此,本研究使用模型聚焦于RA对INS-1细胞的抗糖尿病作用及其机制。将浓度为3 mM的链脲佐菌素(STZ)应用于INS-1细胞4小时以建立糖尿病模型。细胞用不同浓度(1和2.5 μM)的RA预处理24小时。评估细胞活力、葡萄糖刺激的胰岛素分泌(GSIS)、葡萄糖摄取、脂质过氧化、活性氧(ROS)、细胞凋亡以及Bcl-2、NF-κB、IL-1β和PARP的蛋白表达。结果显示,经STZ处理的INS-1细胞表现出细胞活力降低、胰岛素释放减少、胰岛素含量降低、葡萄糖摄取减少以及丙二醛(MDA)和ROS水平升高。用RA预处理的细胞维持了β细胞的功能和形态,抵抗STZ诱导的损伤。此外,RA维持了Bcl-2的高蛋白表达水平以及NF-κB、IL-1β和PARP的低表达水平。总之,RA通过改变NF-κB和Bcl-2信号通路保护β细胞功能免受STZ诱导的损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5dd9/10472240/4e7f5097b679/gr1.jpg

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