Lin Yi, Luo Wangdu, Jiang Bingcai, Lin Qianyi, Tang Min, Li Xiangji, Xie Lin
Department of Ophthalmology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China.
iScience. 2023 Aug 16;26(9):107633. doi: 10.1016/j.isci.2023.107633. eCollection 2023 Sep 15.
Excessive scarring is the main cause of surgical failure in glaucoma filtration surgery. S58 has been shown to have an excellent antifibrotic effect but its duration of action is not sufficient to achieve the desired antiscarring effect. In this study, a light-cured bioadhesive hydrogel composed of GelMA and oxidized dextran (ODex), namely, GelDex, was used to load S58 (GelDex-S58). The microscopic morphology of GelDex-S58 appeared to be a porous structure with good slow-release properties and suitable degradation time. Cell Counting Kit-8, cell scratch and transwell assays showed that GelDex-S58 significantly reduced TGF-β-induced fibroblast proliferation, increased migration and invasion ability. In studies, GelDex-S58 treatment prolonged follicular retention, reduced mean intraocular pressure, and significantly reduced collagen deposition and α-SMA expression levels in the conjunctival tissue compared to treatment with S58 alone. In conclusion, GelDex-S58 could reduce scar formation after glaucoma filtration surgery.
过度瘢痕形成是青光眼滤过手术失败的主要原因。S58已被证明具有出色的抗纤维化作用，但其作用持续时间不足以达到理想的抗瘢痕效果。在本研究中，一种由甲基丙烯酰化明胶（GelMA）和氧化葡聚糖（ODex）组成的光固化生物粘附水凝胶，即GelDex，被用于负载S58（GelDex-S58）。GelDex-S58的微观形态呈多孔结构，具有良好的缓释性能和合适的降解时间。细胞计数试剂盒-8、细胞划痕和Transwell实验表明，GelDex-S58显著降低了转化生长因子-β（TGF-β）诱导的成纤维细胞增殖，提高了迁移和侵袭能力。在研究中，与单独使用S58治疗相比，GelDex-S58治疗延长了滤过泡保留时间，降低了平均眼压，并显著减少了结膜组织中的胶原沉积和α-平滑肌肌动蛋白（α-SMA）表达水平。总之，GelDex-S58可减少青光眼滤过手术后的瘢痕形成。
