Rochard Vincent, Cochard Thierry, Crapart Stéphanie, Delafont Vincent, Moyen Jean-Louis, Héchard Yann, Biet Franck
Laboratoire Ecologie et Biologie des Interactions, UMR Centre National de la Recherche Scientifique 7267, Université de Poitiers, Equipe Microbiologie de l'Eau, F-86073 Poitiers, France.
Institut National de Recherche pour l'Agriculture-INRAE, Université de Tours, ISP, F-37390 Nouzilly, France.
Animals (Basel). 2023 May 27;13(11):1781. doi: 10.3390/ani13111781.
One of the obstacles to eradicating or Johne's Disease (JD) seems to be the persistence of subsp. () in the environment due to its ability to survive alone or vectorized. It has been shown that is widely distributed in soils and water. Previously, we isolated amoebae associated with strains in the environment of bovines from an infected herd. This work aims to verify our working hypothesis, which suggests that amoebae may play a role in the transmission of JD. In this study, we sampled water in the vicinity of herds infected with or () and searched for amoebae and mycobacteria. Live amoebae were recovered from all samples. Among these amoebae, four isolates associated with the presence of mycobacteria were identified and characterized. and other mycobacterial species were detected by qPCR and, in some cases, by culture. This study suggests that amoebae and may be found in the same environment and might represent a risk of exposure of animals to pathogenic mycobacteria. These data open up new perspectives on the control measures to be put in place to prevent contamination by .
根除副结核（JD）的障碍之一似乎是副结核分枝杆菌亚种（Mycobacterium avium subsp. paratuberculosis）能够在环境中单独存活或通过载体传播，从而持续存在。研究表明，副结核分枝杆菌在土壤和水中广泛分布。此前，我们从一个受感染牛群的环境中分离出与副结核分枝杆菌菌株相关的变形虫。这项工作旨在验证我们的工作假设，即变形虫可能在副结核的传播中起作用。在本研究中，我们对感染副结核分枝杆菌或副结核分枝杆菌亚种的牛群附近的水进行采样，并寻找变形虫和分枝杆菌。从所有样本中都回收了活的变形虫。在这些变形虫中，鉴定并表征了四种与分枝杆菌存在相关的分离株。通过qPCR以及在某些情况下通过培养检测到副结核分枝杆菌和其他分枝杆菌物种。这项研究表明，变形虫和副结核分枝杆菌可能存在于同一环境中，并且可能代表动物接触致病性分枝杆菌的风险。这些数据为制定预防副结核分枝杆菌污染的控制措施开辟了新的前景。
