Pillars Roxanne B, Grooms Daniel L, Kaneene John B
Center for Comparative Epidemiology, Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, 48824, USA.
Can Vet J. 2009 Oct;50(10):1039-46.
The objective of this study was to describe the distribution of Mycobacterium avium subsp. paratuberculosis (MAP) in the environment of infected dairy farms over time. Johne's disease (JD) prevalence was monitored annually in 7 Michigan dairy herds. Environmental samples were collected bi-annually and cultured for MAP. Of 731 environmental samples that were cultured, 81 (11%) were positive. The lactating cow floor and manure storage areas were the areas most commonly contaminated, representing 30% and 33% of positive samples, respectively. When herd prevalence was > 2%, MAP was cultured from the lactating cow floor and/or manure storage area 75% of the time. When herd prevalence was < or = 2%, MAP was never cultured from samples collected. For every 1 unit increase in number of positive environmental samples, within herd JD prevalence increased 1.62%. Environmental contamination with MAP is consistent over time on infected dairy farms, and management practices to reduce environmental contamination are warranted.
本研究的目的是描述随着时间推移，副结核分枝杆菌（MAP）在受感染奶牛场环境中的分布情况。每年对密歇根州的7个奶牛群监测约翰氏病（JD）的患病率。每半年采集一次环境样本并进行MAP培养。在731份培养的环境样本中，81份（11%）呈阳性。泌乳牛舍地面和粪便储存区是最常受污染的区域，分别占阳性样本的30%和33%。当牛群患病率>2%时，75%的情况下可从泌乳牛舍地面和/或粪便储存区培养出MAP。当牛群患病率≤2%时，从未从采集的样本中培养出MAP。环境阳性样本数量每增加1个单位，牛群内JD患病率增加1.62%。受感染奶牛场中MAP的环境污染随时间保持一致，因此有必要采取管理措施减少环境污染。
