Zhao Jiu-Xia, Wang Shu, Liu Jiazhi, Jiang Xiao-Dong, Wen Jing, Suo Zhi-Quan, Liu Jie, Zhong Mi-Cai, Wang Qin, Gu Zhirong, Liu Changning, Deng Yunfei, Hu Jin-Yong, Li De-Zhu
CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Plant J. 2023 Dec;116(6):1842-1855. doi: 10.1111/tpj.16452. Epub 2023 Sep 4.
Perennial monocarpic mass flowering represents as a key developmental innovation in flowering time diversity in several biological and economical essential families, such as the woody bamboos and the shrubby Strobilanthes. However, molecular and genetic mechanisms underlying this important biodiversity remain poorly investigated. Here, we generated a full-length transcriptome resource incorporated into the BlueOmics database (http://blueomics.iflora.cn) for two Strobilanthes species, which feature contrasting flowering time behaviors. Using about 112 and 104 Gb Iso-seq reads together with ~185 and ~75 Gb strand-specific RNA seq data, we annotated 80 971 and 79 985 non-redundant full-length transcripts for the perennial polycarpic Strobilanthes tetrasperma and the perennial monocarpic Strobilanthes biocullata, respectively. In S. tetrasperma, we identified 8794 transcripts showing spatiotemporal expression in nine tissues. In leaves and shoot apical meristems at two developmental stages, 977 and 1121 transcripts were differentially accumulated in S. tetrasperma and S. biocullata, respectively. Interestingly, among the 33 transcription factors showing differential expression in S. tetrasperma but without differential expression in S. biocullata, three were involved potentially in the photoperiod and circadian-clock pathway of flowering time regulation (FAR1 RELATED SEQUENCE 12, FRS12; NUCLEAR FACTOR Y A1, NFYA1; PSEUDO-RESPONSE REGULATOR 5, PRR5), hence provides an important clue in deciphering the flowering diversity mechanisms. Our data serve as a key resource for further dissection of molecular and genetic mechanisms underpinning key biological innovations, here, the perennial monocarpic mass flowering.
多年生一次结实的集中开花现象是几个生物学和经济重要科属开花时间多样性的关键发育创新，如木本竹子和灌木状的马蓝属植物。然而，这种重要生物多样性背后的分子和遗传机制仍未得到充分研究。在此，我们为两种开花时间行为不同的马蓝属植物构建了一个整合到BlueOmics数据库（http://blueomics.iflora.cn）中的全长转录组资源。利用约112和104 Gb的Iso-seq reads以及约185和75 Gb的链特异性RNA-seq数据，我们分别注释了多年生多次结实的四子马蓝和多年生一次结实的比氏马蓝80971和79985条非冗余全长转录本。在四子马蓝中，我们鉴定出8794条在九个组织中呈现时空表达的转录本。在两个发育阶段的叶片和茎尖分生组织中，四子马蓝和比氏马蓝分别有977和1121条转录本差异积累。有趣的是，在四子马蓝中差异表达但在比氏马蓝中无差异表达的33个转录因子中，有三个可能参与开花时间调控的光周期和生物钟途径（远红光相关序列12，FRS12；核因子Y A1，NFYA1；伪反应调节因子5，PRR5），从而为破译开花多样性机制提供了重要线索。我们的数据是进一步剖析关键生物学创新（即多年生一次结实的集中开花）背后分子和遗传机制的关键资源。
