Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Guangzhou City Key Laboratory of Subtropical Fruit Trees Outbreak Control, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Pestic Biochem Physiol. 2023 Sep;195:105537. doi: 10.1016/j.pestbp.2023.105537. Epub 2023 Jul 15.
The fall armyworm, Spodoptera frugiperda, is a polyphagous pest worldwide and feeds on many grain and cash crops, which threatens the safety of agriculture and forestry production. Toosendanin (TSN) is a commercial insecticidal active ingredient used to manage various pests in the field and showed adverse effects against S. frugiperda, while the effects of TSN on the larval midguts are not yet known. In this study, the effects of 10 and 20 mg/kg TSN exposures on the larval midguts were analyzed. The structural changes of the larval midgut induced by TSN treatments were also determined by hematoxylin-eosin staining. Besides, TSN treatments also changed the enzyme activities of three digestive enzymes (α-amylase, lipase, and trypsin) and two detoxification enzymes (CarE and GST). A total of 2868 differentially expressed genes (DEGs) were identified by RNA-Seq in the larval midguts with 20 mg/kg TSN treatment, and the DEGs responsible for food digestion and detoxification were further examined. Our findings revealed the preliminary modes of action of TSN on the larval midguts of S. frugiperda, which provide a preliminary rationale for controlling S. frugiperda with TSN in the field.
秋粘虫（Spodoptera frugiperda）是一种世界性的多食性害虫，以许多粮食和经济作物为食，威胁着农业和林业生产的安全。川楝素（TSN）是一种商业性的杀虫活性成分，用于防治田间的各种害虫，对 S. frugiperda 表现出不良影响，而 TSN 对幼虫中肠的影响尚不清楚。在本研究中，分析了 10 和 20 mg/kg TSN 暴露对幼虫中肠的影响。通过苏木精-伊红染色确定 TSN 处理诱导的幼虫中肠的结构变化。此外，TSN 处理还改变了三种消化酶（α-淀粉酶、脂肪酶和胰蛋白酶）和两种解毒酶（CarE 和 GST）的酶活性。用 20 mg/kg TSN 处理幼虫的中肠进行 RNA-Seq 鉴定了 2868 个差异表达基因（DEGs），并进一步研究了与食物消化和解毒相关的 DEGs。我们的研究结果揭示了 TSN 对 S. frugiperda 幼虫中肠的初步作用模式，为田间用 TSN 防治 S. frugiperda 提供了初步的理论依据。
