Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
Department of Cardiology, The Affiliated Hospital of Southwest Medical University, Luzhou, 646000, China.
Sci Data. 2023 Sep 4;10(1):577. doi: 10.1038/s41597-023-02333-6.
The development of the cardiac conduction system (CCS) is essential for correct heart function. However, critical details on the cell types populating the CCS in the mammalian heart during the development remain to be resolved. Using single-cell RNA sequencing, we generated a large dataset of transcriptomes of ~0.5 million individual cells isolated from murine hearts at six successive developmental corresponding to the early, middle and late stages of heart development. The dataset provides a powerful library for studying the development of the heart's CCS and other cardiac components. Our initial analysis identified distinct cell types between 20 to 26 cell types across different stages, of which ten are involved in forming the CCS. Our dataset allows researchers to reuse the datasets for data mining and a wide range of analyses. Collectively, our data add valuable transcriptomic resources for further study of cardiac development, such as gene expression, transcriptional regulation and functional gene activity in developing hearts, particularly the CCS.
心脏传导系统（CCS）的发育对于心脏的正常功能至关重要。然而，哺乳动物心脏中CCS 在发育过程中细胞类型的关键细节仍有待解决。本研究采用单细胞 RNA 测序技术，生成了一个包含约 50 万个分离自 6 个连续发育阶段（对应于心脏发育的早期、中期和晚期）的小鼠心脏细胞转录组的大型数据集。该数据集为研究心脏 CCS 和其他心脏成分的发育提供了一个强大的文库。我们的初步分析在不同阶段之间确定了 20 到 26 种不同的细胞类型，其中 10 种参与形成 CCS。我们的数据集允许研究人员对数据集进行重复使用，以进行数据挖掘和广泛的分析。总的来说，我们的数据为进一步研究心脏发育，如发育心脏中的基因表达、转录调控和功能基因活性，特别是 CCS，提供了有价值的转录组资源。
