Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom (J.A.P., S.A.T.).
Wellcome-MRC Cambridge Stem Cell Institute, Jeffrey Cheah Biomedical Centre, Cambridge Biomedical Campus (J.A.P., S.A.T.), University of Cambridge, United Kingdom.
Circ Res. 2024 Jun 7;134(12):1681-1702. doi: 10.1161/CIRCRESAHA.124.323672. Epub 2024 Jun 6.
Throughout our lifetime, each beat of the heart requires the coordinated action of multiple cardiac cell types. Understanding cardiac cell biology, its intricate microenvironments, and the mechanisms that govern their function in health and disease are crucial to designing novel therapeutical and behavioral interventions. Recent advances in single-cell and spatial omics technologies have significantly propelled this understanding, offering novel insights into the cellular diversity and function and the complex interactions of cardiac tissue. This review provides a comprehensive overview of the cellular landscape of the heart, bridging the gap between suspension-based and emerging in situ approaches, focusing on the experimental and computational challenges, comparative analyses of mouse and human cardiac systems, and the rising contextualization of cardiac cells within their niches. As we explore the heart at this unprecedented resolution, integrating insights from both mouse and human studies will pave the way for novel diagnostic tools and therapeutic interventions, ultimately improving outcomes for patients with cardiovascular diseases.
在我们的一生中，心脏的每一次跳动都需要多种心脏细胞类型的协调作用。了解心脏细胞生物学、其复杂的微环境以及控制其在健康和疾病中功能的机制，对于设计新的治疗和行为干预措施至关重要。单细胞和空间组学技术的最新进展极大地推动了这方面的理解，为心脏的细胞多样性和功能以及心脏组织的复杂相互作用提供了新的见解。本综述提供了心脏细胞景观的全面概述，弥合了基于悬浮和新兴原位方法之间的差距，重点关注实验和计算挑战、小鼠和人类心脏系统的比较分析，以及心脏细胞在其龛位中的上下文化。随着我们以前所未有的分辨率探索心脏，整合来自小鼠和人类研究的见解将为新型诊断工具和治疗干预措施铺平道路，最终改善心血管疾病患者的治疗效果。
