组织中转录组和代谢组的空间多模态分析。
Spatial multimodal analysis of transcriptomes and metabolomes in tissues.
机构信息
Department of Gene Technology, KTH Royal Institute of Technology, Science for Life Laboratory, Stockholm, Sweden.
Department of Pharmaceutical Biosciences, Spatial Mass Spectrometry, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
出版信息
Nat Biotechnol. 2024 Jul;42(7):1046-1050. doi: 10.1038/s41587-023-01937-y. Epub 2023 Sep 4.
We present a spatial omics approach that combines histology, mass spectrometry imaging and spatial transcriptomics to facilitate precise measurements of mRNA transcripts and low-molecular-weight metabolites across tissue regions. The workflow is compatible with commercially available Visium glass slides. We demonstrate the potential of our method using mouse and human brain samples in the context of dopamine and Parkinson's disease.
我们提出了一种空间组学方法，它结合了组织学、质谱成像和空间转录组学，以促进跨组织区域的 mRNA 转录本和低分子量代谢物的精确测量。该工作流程与市售的 Visium 玻片兼容。我们使用多巴胺和帕金森病背景下的小鼠和人脑样本展示了我们方法的潜力。
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