Institute of Pharmaceutical Process, Academy of Nutrition and Health Hubei Province Key Laboratory of Occupational Hazard Identification and Control School of Medicine, Wuhan University of Science and Technology Wuhan, China.
College of Medical Science, Three Gorges University, Yichang, Hubei, China.
Pharm Biol. 2023 Dec;61(1):1401-1412. doi: 10.1080/13880209.2023.2244532.
is the dried rhizome of C.A. Mey. (Araliaceae). Saponins from (SPJ) exhibit anti-oxidative and anti-aging effects.
We evaluated the neuroprotective effects of SPJ on aging rats.
Sprague-Dawley rats (18-months-old) were randomly divided into aging and SPJ groups ( = 8). Five-month-old rats were taken as the adult control ( = 8). The rats were fed a normal chow diet or the SPJ-containing diet (10 or 30 mg/kg) for 4 months. An model was established by d-galactose (d-Gal) in the SH-SY5Y cell line and pretreated with SPJ (25 and 50 µg/mL). The neuroprotection of SPJ was evaluated via Nissl staining, flow cytometry, transmission electron microscopy and Western blotting and .
SPJ improved the neuronal degeneration and mitochondrial morphology that are associated with aging. Meanwhile, SPJ up-regulated the protein levels of mitofusin 2 (Mfn2) and optic atrophy 1 (Opa1) and down-regulated the protein level of dynamin-like protein 1 (Drp1) in the hippocampus of aging rats ( < 0.05 or < 0.01 vs. 22 M). The studies also demonstrated that SPJ attenuated d-Gal-induced cell senescence concomitant with the improvement in mitochondrial function; SPJ, also up-regulated the Mfn2 and Opa1 protein levels, whereas the Drp1 protein level ( < 0.05 or < 0.01 vs. d-Gal group) was down-regulated.
Further research on the elderly population will contribute to the development and utilization of SPJ for the treatment of neurodegenerative disorders.
是伞形科的干燥根茎。从（SPJ）中提取的皂苷具有抗氧化和抗衰老作用。
评价 SPJ 对衰老大鼠的神经保护作用。
将 Sprague-Dawley 大鼠（18 月龄）随机分为衰老组和 SPJ 组（每组 8 只）。5 月龄大鼠作为成年对照组（每组 8 只）。大鼠分别给予正常饲料或含 SPJ 的饲料（10 或 30mg/kg）喂养 4 个月。通过半乳糖（d-Gal）在 SH-SY5Y 细胞系中建立模型，并预先用 SPJ（25 和 50μg/ml）处理。通过尼氏染色、流式细胞术、透射电镜和 Western blot 评估 SPJ 的神经保护作用，并检测相关蛋白。
SPJ 改善了与衰老相关的神经元变性和线粒体形态。同时，SPJ 上调了衰老大鼠海马中融合蛋白 2（Mfn2）和视神经萎缩 1（Opa1）的蛋白水平，下调了动力相关蛋白 1（Drp1）的蛋白水平（与 22M 相比，均 < 0.05 或 < 0.01）。研究还表明，SPJ 可减轻半乳糖诱导的细胞衰老，同时改善线粒体功能；SPJ 还上调了 Mfn2 和 Opa1 的蛋白水平，而下调了 Drp1 蛋白水平（与半乳糖组相比，均 < 0.05 或 < 0.01）。
对老年人的进一步研究将有助于 SPJ 的开发和利用，以治疗神经退行性疾病。
