Saponins from attenuate cognitive impairment in ageing rats through regulating microglial polarisation and autophagy.

CONTEXT

is the dried rhizome of C.A. Mey. (Araliaceae). Saponins from (SPJ) exhibit anti-inflammatory and antioxidative effects.

OBJECTIVE

To explore the neuroprotective effect of SPJ on natural ageing of rat.

MATERIALS AND METHODS

Sprague-Dawley (SD) rats 18-month-old were divided into ageing control, ageing treated with SPJ 10 or 30 mg/kg ( = 8). Five-month-old rats were taken as the adult control ( = 8). Rats were fed regular feed or feed containing SPJ for 4 months. Cognitive level was evaluated by Morris water maze (MWM) test. The mechanisms of SPJ's neuroprotection were evaluated by transmission electron microscope, western blot analysis, and immunofluorescence and .

RESULTS

SPJ attenuated ageing-induced cognitive impairment as indicated by elevated number of times crossing the target platform (from 1.63 to 3.5) and longer time spent in the target platform quadrant (from 1.33 to 1.98). Meanwhile, SPJ improved the morphology of microglia and synapse, and activated M2 microglia polarisation including increased hippocampus levels of CD206 (from 0.98 to 1.47) and YM-1 (from 0.67 to 1.1), and enhanced autophagy-related proteins LC3B (from 0.48 to 0.82), Beclin1 (from 0.32 to 0.51), Atg5 (from 0.22 to 0.89) whereas decreased p62 level (from 0.71 to 0.45) of ageing rats. study also showed that SPJ regulated the microglial polarisation and autophagy.

DISCUSSION AND CONCLUSIONS

SPJ improved cognitive deficits of ageing rats through attenuating microglial inflammation and enhancing microglial autophagy, which could be used to treat neurodegenerative disorders.