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经辐照的卵囊与菊粉佐剂联合使用可诱导肉鸡产生针对感染的强效免疫反应。

Irradiated oocysts in combination with inulin adjuvant-induced potent immune responses against infection in broiler chickens.

作者信息

Falsafi Monireh, Delirezh Nowruz, Safarzadeh Elham, Talebi Alireza, Heidari Zahra

机构信息

Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Department of Medical Microbiology, Parasitology and Immunology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.

出版信息

Vet Res Forum. 2023;14(8):423-429. doi: 10.30466/vrf.2023.563143.3675. Epub 2023 Aug 15.

Abstract

Coccidiosis is the leading parasitic disease in poultry. One of the most critical species, , lives inside the cecal epithelial cells and induces bloody coccidiosis. The present study evaluated the effect of radiation-attenuated oocytes mixed with inulin adjuvant on broiler chicken. Initially, the effect of irradiation on oocyst attenuation was confirmed. Then, one-day-old broilers (n = 90) were divided into nine groups on seven days of age as follow: Group 1 (400 attenuated oocysts + 1.00 mg of adjuvant), group 2 (400 attenuated oocysts + 0.50 mg adjuvant), group 3 (200 attenuated oocysts + 1.00 mg of adjuvant), group 4 (200 attenuated oocysts + 0.50 mg adjuvant), group 5 (1.00 mg adjuvant), group 6 (400 attenuated oocysts), group 7 (commercial vaccine), group 8 (negative control) and group 9 (blank). On day 21, we performed a challenge with oocytes and investigated oocyst output and average weekly weight throughout the study. At the end of the study, we evaluated macroscopic lesion, histology, cytokine level and leukogram status. The results showed a statistically significant difference among groups. Furthermore, the optimal dose was 400 irradiated oocysts and 1.00 mg of inulin. Moreover, an X-ray could reduce the virulence of oocytes. Inulin alone or combined with attenuated oocysts showed an acceptable effect on evaluated parameters.

摘要

球虫病是家禽中主要的寄生虫病。其中最关键的一个种类,寄生于盲肠上皮细胞内并引发出血性球虫病。本研究评估了辐射减毒的卵囊与菊粉佐剂混合对肉鸡的影响。首先,确认了辐照对卵囊减毒的效果。然后,将1日龄的肉鸡(n = 90)在7日龄时分为9组,如下:第1组(400个减毒卵囊 + 1.00毫克佐剂),第2组(400个减毒卵囊 + 0.50毫克佐剂),第3组(200个减毒卵囊 + 1.00毫克佐剂),第4组(200个减毒卵囊 + 0.50毫克佐剂),第5组(1.00毫克佐剂),第6组(400个减毒卵囊),第7组(商业疫苗),第8组(阴性对照)和第9组(空白)。在第21天,用卵囊进行攻毒,并在整个研究过程中调查卵囊排出量和平均每周体重。在研究结束时,我们评估了宏观病变、组织学、细胞因子水平和血常规状态。结果显示各组之间存在统计学上的显著差异。此外,最佳剂量是400个辐照卵囊和1.00毫克菊粉。而且,X射线可以降低卵囊的毒力。单独的菊粉或与减毒卵囊联合使用对评估参数显示出可接受的效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/054c/10475167/af95fd14b091/vrf-14-423-g001.jpg

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