Wang Hua, Sun Yang, Zhou Yuan, Liu Yujie, Chen Shuo, Sun Wenbo, Zhang Zidong, Guo Junqing, Yang Cheng, Li Zhengping, Chen Lei
Department of Life Sciences, Shandong Normal University, 1 Daxue Road, Changqing District, Jinan, Shandong Province, 250014, PR China.
Department of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, PR China.
Biosens Bioelectron. 2023 Nov 15;240:115637. doi: 10.1016/j.bios.2023.115637. Epub 2023 Aug 25.
At present, the 100% case fatality and the cross-infection of virus strains make the ASFV 's harm to society continue to expand. The absence of an effective commercial vaccine poses early detection remains the most effective means of curbing ASFV infection. Here, we report a cascaded detection platform based on the CRISPR-Cas12a system combined with graphene field-effect transistor sensors. The cascade platform could detect ASFV as low as 0.5 aM within 30 min and achieve typing of wild and vaccine strains of ASFV in a single detection system. The evaluation of 16 clinical samples proved that, compared with the gold standard Real-time PCR method, this platform has outstanding advantages in sensitivity, specificity and typing. Combining CRISPR-Cas12a's high specificity with the bipolar electric field effect of graphene field-effect transistor, the cascade platform is expected to achieve clinical application in the field of DNA disease detection, and provides a new direction for multi-strain disease typing.
目前，非洲猪瘟病毒（ASFV）100%的致死率以及毒株的交叉感染致使其对社会的危害不断扩大。由于缺乏有效的商用疫苗，早期检测仍是遏制ASFV感染的最有效手段。在此，我们报道了一种基于CRISPR-Cas12a系统与石墨烯场效应晶体管传感器相结合的级联检测平台。该级联平台能够在30分钟内检测低至0.5阿摩尔的ASFV，并在单一检测系统中实现ASFV野生株和疫苗株的分型。对16份临床样本的评估证明，与金标准实时荧光定量聚合酶链反应（Real-time PCR）方法相比，该平台在灵敏度、特异性和分型方面具有突出优势。该级联平台将CRISPR-Cas12a的高特异性与石墨烯场效应晶体管的双极电场效应相结合，有望在DNA疾病检测领域实现临床应用，并为多毒株疾病分型提供新方向。
