Harvey A R, Warton S S
Anat Embryol (Berl). 1986;174(3):361-7. doi: 10.1007/BF00698786.
The Golgi technique has been used to examine the morphology of neurons within tectal transplants. Embryonic tectal tissue was transplanted to the midbrain of newborn rats. Four to eight months later, host animals were decapitated under anaesthesia, the unfixed brains removed and processed by Golgi-Cox impregnation. In tectal grafts, different types of neuron were recognized on the basis of the size and shape of their somata and the morphology of their dendritic trees. Neuronal types found in transplants resembled cell classes found in normal rat superior colliculus (SC). Neurons characteristic of the superficial collicular layers such as marginal, ganglion type I, stellate and horizontal cells and multipolar cells typical of the deeper collicular layers were identified in the transplants. Compared with normal cells, grafted neurons often had smaller dendritic fields and fewer dendritic spines. No laminar organization was discernable in the grafts and there was commonly no preferential orientation of perikarya or dendrites. Small cells with similar dendritic morphology were sometimes found grouped together in patches within the graft neuropil. These patches resembled cytologically and histochemically distinct areas described in previous studies and may represent areas homologous to the superficial layers of normal SC.
高尔基氏技术已被用于研究顶盖移植体内神经元的形态。将胚胎期的顶盖组织移植到新生大鼠的中脑。4至8个月后，在麻醉状态下将宿主动物断头，取出未固定的大脑并通过高尔基-考克斯浸染法进行处理。在顶盖移植物中，根据神经元胞体的大小和形状以及其树突树的形态识别出不同类型的神经元。移植中发现的神经元类型类似于正常大鼠上丘（SC）中发现的细胞类别。在移植物中鉴定出了浅丘层特有的神经元，如边缘细胞、I型神经节细胞、星状细胞和水平细胞，以及深丘层特有的多极细胞。与正常细胞相比，移植的神经元通常具有较小的树突野和较少的树突棘。在移植物中无法辨别出分层结构，并且通常也没有核周体或树突的优先取向。有时会发现具有相似树突形态的小细胞在移植物神经毡内聚集成斑块。这些斑块在细胞学和组织化学上类似于先前研究中描述的不同区域，可能代表与正常SC浅层同源的区域。
