Warton S S, Jones D G
Exp Brain Res. 1985;58(3):490-502. doi: 10.1007/BF00235865.
The postnatal development of the superficial (optic) layers of the rat superior colliculus has been studied using Klüver-Barrera staining and Golgi impregnation in rats aged 3-45 days. The Klüver-Barrera staining reveals that the SC of 3 day old rats is morphologically immature with no obvious lamination. It contains densely packed cells of uniform size. The packing density of the cells gradually decreases between 9 and 15 days as the thickness of the layers increases. The first myelinated fibres in the SC appear at 15 days but the stratum opticum is still not recognizable. By 30 days, the SC has a distinctly laminated appearance, but the thickness of the superficial layers continues to increase until day 45 postnatal. Golgi-Cox impregnation displays the range of neuronal types in the superficial layers of the SC previously described by Langer and Lund (1974). Using the morphological criteria of these authors for classification of the neurons, the developmental changes of the marginal cells, horizontal cells, ganglion cells types I, II, III and stellate cells have been followed. The SC of 3 day old rats contains immature neurons; only a few larger cells have branched dendrites. In 9 days old SC the neuronal types present in the adult are recognizable, although their appearances are still immature. By 15 days neurons have adult-looking dendritic trees but dendritic growth continues beyond 30 days. The visual part of the SC has a protracted period of postnatal development, the sequence of developmental changes being similar for the different types of collicular neurons. Features common to development are the increasing size of neuronal somata, the increasing length of dendrites and the acquisition of a complex pattern of dendritic arborization. Larger cells appear to commence development earlier than small cells, although the rate of developmental changes is different for each of the various types of collicular neurons.
利用Klüver-Barrera染色法和高尔基浸染法，对3至45日龄大鼠上丘浅层（视层）的产后发育进行了研究。Klüver-Barrera染色显示，3日龄大鼠的上丘在形态上不成熟，没有明显的分层。它包含大小均匀、紧密排列的细胞。随着层厚度的增加，细胞的堆积密度在9至15天之间逐渐降低。上丘中第一批有髓纤维出现在15天，但视层仍无法辨认。到30天时，上丘呈现出明显的分层外观，但浅层的厚度在产后45天之前持续增加。高尔基-考克斯浸染法显示了上丘浅层中先前由兰格和伦德（1974年）描述的神经元类型范围。根据这些作者对神经元分类的形态学标准，追踪了边缘细胞、水平细胞、I型、II型、III型神经节细胞和星形细胞的发育变化。3日龄大鼠的上丘包含未成熟的神经元；只有少数较大的细胞有分支的树突。在9日龄的上丘中，可以识别出成体中存在的神经元类型，尽管它们的外观仍然不成熟。到15天时，神经元具有类似成体的树突树，但树突生长会持续到30天以后。上丘的视觉部分有一个延长的产后发育时期，不同类型的丘神经元发育变化的顺序相似。发育的共同特征是神经元胞体增大、树突长度增加以及获得复杂的树突分支模式。较大的细胞似乎比较小的细胞更早开始发育，尽管各种类型的丘神经元发育变化的速度各不相同。
