Institute of Aquatic Economic Animals and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Biology Department, School of Life Sciences, Sun Yat-Sen University, Guangzhou, People's Republic of China.
Fish Physiol Biochem. 2023 Oct;49(5):983-1003. doi: 10.1007/s10695-023-01237-x. Epub 2023 Sep 5.
The neuropeptide B/W signaling system is composed of neuropeptide B (NPB), neuropeptide W (NPW), and two cognate receptors, NPBWR1 and NPBWR2, which are involved in diverse physiological processes, including the central regulation of neuroendocrine axes in vertebrates. The components of this signaling system are not well conserved during vertebrate evolution, implicating its functional diversity. The present study characterized the ricefield eel neuropeptide B/W system, generated a specific antiserum against the neuropeptide B/W receptor, and examined the potential roles of the system in the regulation of adenohypophysial functions. The ricefield eel genome contains npba, npbb, and npbwr2b but lacks the npw, npbwr1, and npbwr2a genes. The loss of npw and npbwr1 probably occurred at the base of ray-finned fish radiation and that of npbwr2a species specifically in ray-finned fish. Npba and npbb genes are produced through whole-genome duplication (WGD) in ray-finned fish. The ricefield eel npba was expressed in the brain and some peripheral tissues, while npbb was predominantly expressed in the brain. The ricefield eel npbwr2b was also expressed in the brain and in some peripheral tissues, such as the pituitary, gonad, heart, and eye. Immunoreactive Npbwr2b was shown to be localized to Lh and Fsh cells but not to Gh or Prl cells in the pituitary of ricefield eels. Npba upregulated the expression of fshb and cga but not lhb mRNA in pituitary fragments of ricefield eels cultured in vitro. The results of the present study suggest that the NPB system of ricefield eels may be involved in the neuroendocrine regulation of reproduction.
神经肽 B/W 信号系统由神经肽 B（NPB）、神经肽 W（NPW）和两个同源受体 NPBWR1 和 NPBWR2 组成，参与多种生理过程，包括脊椎动物中枢神经内分泌轴的调节。该信号系统的组成在脊椎动物进化过程中没有很好地保守，暗示其功能多样性。本研究对稻田鳗神经肽 B/W 系统进行了特征描述，生成了针对神经肽 B/W 受体的特异性抗血清，并研究了该系统在调节腺垂体功能中的潜在作用。稻田鳗基因组包含 npba、npbb 和 npbwr2b，但缺乏 npw、npbwr1 和 npbwr2a 基因。npw 和 npbwr1 的缺失可能发生在射线鳍鱼类辐射的基础上，而 npbwr2a 基因的缺失则发生在射线鳍鱼类中。npba 和 npbb 基因是通过射线鳍鱼类的全基因组复制（WGD）产生的。稻田鳗 npba 在大脑和一些外周组织中表达，而 npbb 主要在大脑中表达。稻田鳗 npbwr2b 也在大脑和一些外周组织中表达，如垂体、性腺、心脏和眼睛。免疫反应性 Npbwr2b 被证明定位于稻田鳗垂体中的 Lh 和 Fsh 细胞，但不在 Gh 或 Prl 细胞中。npba 上调了体外培养的稻田鳗垂体片段中 fshb 和 cga 的表达，但不上调 lhb mRNA 的表达。本研究结果表明，稻田鳗的 NPB 系统可能参与生殖的神经内分泌调节。
