一种大规模并行评估 CRISPR 脱靶的方法。
A massively parallel approach for assessing CRISPR off-targets .
机构信息
Zhejiang Lab, Hangzhou, Zhejiang 311121, China.
Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou 310029, China.
出版信息
Cell Rep Methods. 2023 Aug 28;3(8):100561. doi: 10.1016/j.crmeth.2023.100561.
In a recent issue of , Tian et al. present AID-seq, an approach that enables massively parallel identification of off-targets for different CRISPR nucleases . By using a pooled strategy to simultaneously identify the on-/off-targets of multiple gRNAs, the authors could screen the most efficient and safe gRNA candidates.
在最近一期的 杂志中，Tian 等人提出了 AID-seq 方法，该方法能够大规模平行鉴定不同 CRISPR 核酸酶的脱靶。通过使用池化策略同时鉴定多个 gRNA 的靶上/靶外，作者可以筛选出最有效和安全的 gRNA 候选物。
Zotero 插件