Mashimo Tomoji
Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho Sakyo-ku, Kyoto, 606-8501, Japan.
Dev Growth Differ. 2014 Jan;56(1):46-52. doi: 10.1111/dgd.12110. Epub 2013 Dec 27.
The laboratory rat has been widely used as an animal model in biomedical science for more than 150 years. Applying zinc-finger nucleases or transcription activator-like effector nucleases to rat embryos via microinjection is an efficient genome editing tool for generating targeted knockout rats. Recently, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated endonucleases have been used as an effective tool for precise and multiplex genome editing in mice and rats. In this review, the advantages and disadvantages of these site-specific nuclease technologies for genetic analysis and manipulation in rats are discussed.
在生物医学科学领域，实验大鼠作为动物模型已被广泛应用了150多年。通过显微注射将锌指核酸酶或转录激活样效应因子核酸酶应用于大鼠胚胎，是一种用于生成靶向敲除大鼠的高效基因组编辑工具。最近，成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关内切核酸酶已被用作在小鼠和大鼠中进行精确和多重基因组编辑的有效工具。在这篇综述中，讨论了这些位点特异性核酸酶技术在大鼠基因分析和操作中的优缺点。
