Eichenbaum J D, Lavach J D, Gould D H, Severin G A, Paulsen M E, Jones R L
Am J Vet Res. 1986 Sep;47(9):1952-5.
Fourteen limbal biopsy specimens from 11 dogs with chronic superficial keratitis (CSK) were examined histologically and immunohistochemically. Ten of the 14 specimens had corneal epithelial hyperplasia and/or atrophy. Eleven of the 14 specimens had thickened epithelial basement membranes. Each specimen had cellular infiltration and lamellar disruption of the stroma. An avidin-biotin immunoperoxidase complex stain was used to detect immunoglobulin (Ig) deposition. Twelve of the 14 specimens stained positive for Ig. The staining pattern was consistent and characterized by diffuse deposition of stain in the superficial conjunctival stroma near the limbus. Four of the 12 Ig-positive specimens also stained positive in the superficial corneal stroma with 1 of these 4 also staining positive along the epithelial cell basement membrane. The diffuse pattern of stain deposition and the absence of staining of specific epithelial structures indicated that CSK is not a classical autoimmune disease similar to any disease in the pemphigus group or similar to systemic lupus erythematosus. Although the results may implicate CSK as an immune-mediated disease, nonspecific factors could not be ruled out.
对11只患有慢性浅层角膜炎（CSK）的犬的14个角膜缘活检标本进行了组织学和免疫组织化学检查。14个标本中有10个出现角膜上皮增生和/或萎缩。14个标本中有11个上皮基底膜增厚。每个标本均有细胞浸润和基质板层破坏。采用抗生物素蛋白-生物素免疫过氧化物酶复合物染色法检测免疫球蛋白（Ig）沉积。14个标本中有12个Ig染色呈阳性。染色模式一致，其特征是在角膜缘附近的浅层结膜基质中染色呈弥漫性沉积。12个Ig阳性标本中有4个在角膜浅层基质中也呈阳性染色，其中1个在沿上皮细胞基底膜处也呈阳性染色。染色沉积的弥漫模式以及特定上皮结构无染色表明，CSK并非类似于天疱疮组中任何疾病或系统性红斑狼疮的典型自身免疫性疾病。尽管结果可能提示CSK是一种免疫介导性疾病，但不能排除非特异性因素。
