Cytopeutics Sdn Bhd, Suite 2-3 2nd floor, Bio-X Centre, Persiaran Cyberpoint Selatan, Cyber 8, 63000, Cyberjaya, Selangor, Malaysia.
M. Kandiah Faculty of Medicine & Health Sciences (MK FMHS), Universiti Tunku Abdul Rahman Sungai Long City Campus, Jalan Sungai Long, Bandar Sungai Long, Cheras, 43000, Kajang, Selangor, Malaysia.
Regen Med. 2023 Nov;18(11):839-856. doi: 10.2217/rme-2023-0085. Epub 2023 Sep 6.
To profile and study the proteins responsible for the beneficial effect of the TNF-α-induced human umbilical cord mesenchymal stem cells (hUCMSCs) secretome in wound healing. The hUCMSCs secretome was generated with (induced) or without (uninduced) TNF-α and was subsequently analyzed by liquid chromatography-mass spectrometry, immunoassay and scratch assay. Proteomic analysis revealed approximately 260 proteins, including 51 and 55 unique proteins in the induced and uninduced secretomes, respectively. Gene ontology analysis disclosed that differential proteins in the induced secretome mainly involved inflammation-related terms. The induced secretome, consisting of higher levels of FGFb, VEGF, PDGF and IL-6, significantly accelerated wound closure and enhanced MMP-13 secretion in HaCaT keratinocytes. The secretome from induced hUCMSCs includes factors that promote wound closure.
为了分析和研究 TNF-α 诱导的人脐带间充质干细胞（hUCMSCs）分泌组在伤口愈合中有益作用的相关蛋白。使用（诱导）或不使用（未诱导）TNF-α 生成 hUCMSCs 分泌组，随后通过液相色谱-质谱、免疫测定和划痕实验进行分析。蛋白质组学分析显示约有 260 种蛋白质，其中诱导和未诱导分泌组分别有 51 种和 55 种独特蛋白质。GO 分析显示，诱导分泌组中的差异蛋白主要涉及炎症相关术语。诱导分泌组包含更高水平的 FGFb、VEGF、PDGF 和 IL-6，可显著加速 HaCaT 角质形成细胞的伤口闭合，并增强 MMP-13 的分泌。诱导的 hUCMSCs 分泌组包含促进伤口闭合的因子。
