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Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) and ADMSC-Derived Secretome Expedited Wound Healing in a Rodent Model - A Preliminary Study.

作者信息

Ma Hui, Lam Ping Kuen, Siu Wing Sum, Tong Cindy See Wai, Lo Kin Ki Yan, Koon Chi Man, Wu Xiao Xiao, Li Xiang, Cheng Wen, Shum Wai Ting, Leung Ping Chung

机构信息

Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, Special Administrative Region of China.

State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, Hong Kong, Special Administrative Region of China.

出版信息

Clin Cosmet Investig Dermatol. 2021 Jun 30;14:753-764. doi: 10.2147/CCID.S298105. eCollection 2021.

DOI:10.2147/CCID.S298105
PMID:34234501
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8255652/
Abstract

INTRODUCTION: The biological role of mesenchymal stem cells (MSCs) in wound healing has been demonstrated. However, there were limited studies on the healing effect of secretome which consists of many biological factors secreted by MSCs. In this study, we aimed to compare the therapeutic effects of secretome with MSCs on facilitating wound healing. METHODS: Green fluorescent protein labelled adipose-derived MSCs (GFP-ADMSCs) or secretome was injected in the full-thickness skin excision model on SD rats. The wound healing process was evaluated by calculating the healing rate and the histological examinations on skin biopsy. The cell viability, proliferation and mobility of the rat dermal fibroblasts were compared after different treatments. The inflammatory response in macrophages was indicated by the level of nitric oxide (NO) and inflammatory cytokines through NO assay and ELISA. RESULTS: On day 5 and day 14, both MSCs and secretome accelerated the wound healing, secretome further enhanced the process. GFP-MSCs were detected 10 days after transplantation. The level of IL-6 and TNF-α in blood was reduced after MSCs and secretome treatments. The expressions of VEGF and PCNA were increased after treatment, higher intensity of VEGF was observed in secretome-injected tissue. The concentrations of total protein and VEGF in secretome were 2.2 ± 0.5 mg/mL and 882.0 ± 72.7 pg/mL, respectively. The cell viability and proliferation of FR were promoted significantly after the treatment. The scratch test showed that secretome accelerated the wound healing speed. Secretome reduced the metabolism of macrophages remarkably, but it did not decrease the level of macrophage-secreted NO. The expression of the pro-inflammatory cytokines (IL-6, MCP-1 and TNF-α) was downregulated significantly. CONCLUSION: Our study indicated both MSCs and MSCs-derived secretome enhanced the wound healing process in early phase. Secretome further promoted the healing effects through promoting the fibroblast proliferation and migration and suppressing the inflammatory response.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/a474056be758/CCID-14-753-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/1f80b9597fde/CCID-14-753-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/3cfae9d871da/CCID-14-753-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/f5c3596842fb/CCID-14-753-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/11ded8915809/CCID-14-753-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/4d373d98eed2/CCID-14-753-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/a474056be758/CCID-14-753-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/1f80b9597fde/CCID-14-753-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/3cfae9d871da/CCID-14-753-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/f5c3596842fb/CCID-14-753-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/11ded8915809/CCID-14-753-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/4d373d98eed2/CCID-14-753-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b07f/8255652/a474056be758/CCID-14-753-g0006.jpg

相似文献

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Adipose Tissue-Derived Mesenchymal Stem Cells (ADMSCs) and ADMSC-Derived Secretome Expedited Wound Healing in a Rodent Model - A Preliminary Study.

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[7]
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引用本文的文献

[1]
Enhancing Wound Healing Through Secretome-Loaded 3D-Printed Biomaterials.

Gels. 2025-6-20

[2]
Innovative Strategies: Use of Stromal Cell-Derived Secretome for Chronic Wound Therapy.

Int J Mol Sci. 2025-6-11

[3]
Review on Current Advancements in Facilitation of Burn Wound Healing.

Bioengineering (Basel). 2025-4-18

[4]
The paradigm of stem cell secretome in tissue repair and regeneration: Present and future perspectives.

Wound Repair Regen. 2025

[5]
Cellular In Vitro Responses Induced by Human Mesenchymal Stem/Stromal Cell-Derived Extracellular Vesicles Obtained from Suspension Culture.

Int J Mol Sci. 2024-7-11

[6]
The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration.

Biomedicines. 2024-3-27

[7]
Unlocking the versatile potential: Adipose-derived mesenchymal stem cells in ocular surface reconstruction and oculoplastics.

World J Stem Cells. 2024-2-26

[8]
Adipose-Derived Stem Cells' Secretome Attenuates Lesion Size and Parasite Loading in Leishmaniasis Caused by in Mice.

Iran J Med Sci. 2024-2

[9]
The Potential of MSC-Based Cell-Free Therapy in Wound Healing-A Thorough Literature Review.

Int J Mol Sci. 2023-5-27

[10]
Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects.

Organogenesis. 2023-12-31

本文引用的文献

[1]
The neuroprotection of hypoxic adipose tissue-derived mesenchymal stem cells in experimental traumatic brain injury.

Cell Transplant. 2019-6-12

[2]
Human Wounds and Its Burden: An Updated Compendium of Estimates.

Adv Wound Care (New Rochelle). 2019-2-1

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J Cell Physiol. 2018-12-4

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Artif Cells Nanomed Biotechnol. 2018-2-15

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Dermal fibroblasts-A heterogeneous population with regulatory function in wound healing.

Cytokine Growth Factor Rev. 2018-2-1

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An Economic Evaluation of the Impact, Cost, and Medicare Policy Implications of Chronic Nonhealing Wounds.

Value Health. 2018-1

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Mediators Inflamm. 2017-10-29

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Wound healing.

J Chin Med Assoc. 2017-11-21

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MSC-derived cytokines repair radiation-induced intra-villi microvascular injury.

Oncotarget. 2017-9-23

[10]
Evaluation of bone marrow derived mesenchymal stem cells for full-thickness wound healing in comparison to tissue engineered chitosan scaffold in rabbit.

Tissue Cell. 2017-2

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