Department of Clinical Immunology, Section 2034, Rigshospitalet, Copenhagen, Denmark.
Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark.
Vox Sang. 2023 Nov;118(11):980-987. doi: 10.1111/vox.13524. Epub 2023 Sep 6.
For 5 years, routine genotyping has been performed for selected blood groups of blood donors in the Copenhagen Capital Region, Denmark. The result is summarized in the following.
Genotyping was carried out by an external service provider using the competitive allele specific PCR (KASP) technology. The genotypes were returned to the blood bank and translated into phenotypes by a proprietary IT application.
In total, 65 alleles from 16 blood group systems (ABO, MNS, Rh, Lutheran, Kell, Duffy, Kidd, Diego, Yt, Dombrock, Colton, Landsteiner-Wiener, Cromer, Knops, Vel, secretor status) and the HPA1, HPA5 and HPA15 antigens were interrogated. After translation, phenotypes were imported into the laboratory information management system of the blood bank. The results from 31,538 genotyped blood donors were used to calculate the allele frequencies for a Danish blood donor population. ABO genotyping was done for sample ID purposes. Determination of the 1061delC single nucleotide polymorphism (SNP) (NM_020469.2), most frequently characteristic of ABO*A2, was validated against a series of 1287 samples with Dolichos biflorus lectin determination of the A1 phenotype.
We report allele frequencies and phenotype frequencies for 16 blood groups from a total of 31,538 genotyped blood donors. Blood products were supplied from a total of 64,312 active blood donors, and of these active blood donors 25,396 (39.5%) were genotyped. These donors represent a valuable resource for patient treatment. This genotyping has enabled the provision of rare genotyped donor blood for patients with alloantibodies and rare reagent cells for serology.
在丹麦哥本哈根首都大区，对献血者的部分选定血型进行了 5 年的常规基因分型。现将结果总结如下。
采用外部服务提供商的竞争性等位基因特异性 PCR（KASP）技术进行基因分型。基因型结果反馈给血库，并由专有的 IT 应用程序转换为表型。
共检测了 16 个血型系统（ABO、MNS、Rh、Lutheran、Kell、Duffy、Kidd、Diego、Yt、Dombrock、Colton、Landsteiner-Wiener、Cromer、Knops、Vel、分泌状态）的 65 个等位基因和 HPA1、HPA5 和 HPA15 抗原。表型经过翻译后被导入血库的实验室信息管理系统。利用 31538 名基因分型献血者的结果计算丹麦献血者人群的等位基因频率。ABO 基因分型用于样本 ID 目的。对最常见于 ABO*A2 的 1061delC 单核苷酸多态性（SNP）（NM_020469.2）的检测，通过与 1287 个样本的 Dolichos biflorus 凝集素测定 A1 表型进行了验证。
我们报告了总共 31538 名基因分型献血者的 16 个血型的等位基因频率和表型频率。从总共 64312 名活跃献血者中供应血液产品，其中 25396 名（39.5%）进行了基因分型。这些献血者为患者治疗提供了宝贵的资源。这种基因分型使我们能够为具有同种抗体的患者提供稀有基因分型的供者血液和稀有试剂细胞用于血清学检测。
