Phillips R A, Melchers F
J Immunol. 1979 Apr;122(4):1473-5.
When pre-B cells are isolated from bone marrow and incubated in vitro for several days, they spontaneously differentiate into LPS-reactive B cells. These newly produced B cells do not respond to another B cell mitogen, lipoprotein (Lp). The lack of Lp reactivity of newly produced B cells represents a qualitative difference between these cells and B cells in peripheral tissues that respond equally well to both LPS and Lp. Thus, responsiveness to different mitogens provides unique markers to identify B cells at early stages of development.
当从骨髓中分离出前B细胞并在体外培养数天时，它们会自发分化为对脂多糖（LPS）有反应的B细胞。这些新产生的B细胞对另一种B细胞有丝分裂原脂蛋白（Lp）无反应。新产生的B细胞缺乏Lp反应性，这代表了这些细胞与外周组织中对LPS和Lp反应同样良好的B细胞之间的质的差异。因此，对不同有丝分裂原的反应性提供了独特的标志物，用于识别发育早期的B细胞。
