Hand C W, Baldwin D, Moore R A, Allen M C, McQuay H J
Ann Clin Biochem. 1986 Jan;23 ( Pt 1):47-53. doi: 10.1177/000456328602300105.
Quantitative analysis of potent opiate drugs in plasma by radioimmunoassay is potentially inaccurate because of the occurrence of cross-reacting metabolites. This paper describes the chemical synthesis of buprenorphine-3-O-glucuronide, a metabolite of buprenorphine, and an extraction procedure coupled with radioimmunoassay which allows the sensitive and specific measurement of buprenorphine using an iodinated buprenorphine derivative. The measurement of extracted and unextracted samples using two different antisera allowed investigation of the metabolism of buprenorphine. In four patients who had taken sublingual buprenorphine for at least one month, N-dealkyl buprenorphine was present in similar concentrations to those of buprenorphine, while buprenorphine-3-O-glucuronide was present in two to three times those concentrations.
由于交叉反应代谢物的存在，通过放射免疫分析法对血浆中强效阿片类药物进行定量分析可能不准确。本文描述了丁丙诺啡代谢物丁丙诺啡 - 3 - O - 葡萄糖醛酸苷的化学合成，以及一种与放射免疫分析相结合的提取方法，该方法允许使用碘化丁丙诺啡衍生物对丁丙诺啡进行灵敏且特异的测量。使用两种不同抗血清对提取和未提取的样本进行测量，从而能够研究丁丙诺啡的代谢情况。在四名舌下含服丁丙诺啡至少一个月的患者中，N - 去烷基丁丙诺啡的浓度与丁丙诺啡相似，而丁丙诺啡 - 3 - O - 葡萄糖醛酸苷的浓度则是丁丙诺啡的两到三倍。
