Bartlett A J, Lloyd-Jones J G, Rance M J, Flockhart I R, Dockray G J, Bennett M R, Moore R A
Eur J Clin Pharmacol. 1980 Oct;18(4):339-45. doi: 10.1007/BF00561392.
Antisera to buprenorphine were obtained in rabbits immunised with 3-0-carboxymethylbuprenorphine and N-hemisuccinyl-norbuprenorphine conjugated to bovine serum albumin. Using the latter antiserum and tritium labelled buprenorphine a radioimmunoassay have good accuracy and precision was developed for concentrations as low as 50 picograms in 1 ml of plasma. The N-hemisuccinyl antiserum crossreacted with norbuprenorphine, and the 3-0-glucuronide conjugate with the 3-0-carboxymethyl antiserum. Cross-reactivity of both antisera to other pharmacologically related compounds was negligible. The assay was employed to determine plasma buprenorphine concentration following its parenteral administration to dog and man.
用与牛血清白蛋白偶联的3 - O - 羧甲基丁丙诺啡和N - 半琥珀酰去甲丁丙诺啡免疫家兔，获得了丁丙诺啡抗血清。使用后一种抗血清和氚标记的丁丙诺啡，开发了一种放射免疫分析法，该方法对于1毫升血浆中低至50皮克的浓度具有良好的准确性和精密度。N - 半琥珀酰抗血清与去甲丁丙诺啡交叉反应，3 - O - 羧甲基抗血清与3 - O - 葡萄糖醛酸结合物交叉反应。两种抗血清与其他药理相关化合物的交叉反应可忽略不计。该分析方法用于测定犬和人经肠胃外给药后血浆中丁丙诺啡的浓度。
