探讨阿联酋北部地区妇女巴氏涂片样本中人乳头瘤病毒（HPV）基因型的流行情况：基于 HPV Direct Flow CHIP 系统的初步研究。
Exploring the prevalence of Human Papillomavirus (HPV) genotypes in PAP smear samples of women in northern region of United Arab Emirates (UAE): HPV Direct Flow CHIP system-based pilot study.
机构信息
Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman, United Arab Emirates.
Thumbay Research Institute for Precision Medicine, College of Medicine, Gulf Medical University, Ajman, United Arab Emirates.
出版信息
PLoS One. 2023 Sep 6;18(9):e0286889. doi: 10.1371/journal.pone.0286889. eCollection 2023.
OBJECTIVE
The aim of this study was to explore the prevalence of low and high-risk HPV genotypes in PAP smear samples of women in northern region of the UAE using HPV direct flow CHIP method.
METHODS
A cross-sectional retrospective study was conducted between September 2021 to April 2022. A total of 104 liquid-based cervical cytology samples were obtained from women aged 20-59 years attending the Gynaecology out-patient department of Thumbay University Hospital and other hospitals of Northern Emirates of UAE, processed for the routine cytological examination to identify and differentiate morphological changes of the PAP smear samples. HPV genotyping was performed using HPV direct flow CHIP method.
RESULTS
In total, 112 HPV genotypes were detected in 63 women (60.57%) included 18 abnormal cytological and 45 normal epithelial samples. 63 LR and 49 HR HPV genotypes were identified in all the 63 positive samples. Highest rate of infection with multiple LR and HR HPV genotypes were detected in women aged 40-49 years (25.9%) and 20-29 years (23.5%). Infection by HPV6 (13.46%), HPV11 (9.61%), HPV16 (9.61%), HPV62/81 (7.69%) and HPV45 (7.69%) were the most common genotypes. A moderate increase than expected incidence of HPV45 and 62/81 (7.69%) were detected. Co-infection with multiple low and high-risk genotypes is present in 20.2% cases; in that, HPV6 (15.9%) was the most common followed by HPV62/81 (12.7%) and HPV16 (11.11%). The prevalence of HPV18 was found to be 1.6%.
CONCLUSION
The genotypes 6, 45, 16, 11, 67, 62/81 were the most common HPV infections in the women between the age group of 21 and 59-years-old. A moderate increase of HPV45, 62/81 and much less prevalence of HPV18 were detected in the study population. 43.27% of the normal epithelia were positive to different low and high-risk HPV genotypes. This finding highlights the importance of molecular genotyping of HPV to emphasize the cervical screening triage.
目的
本研究旨在使用 HPV 直接流芯片法探讨阿联酋北部地区女性巴氏涂片样本中低危和高危 HPV 基因型的流行情况。
方法
这是一项 2021 年 9 月至 2022 年 4 月进行的横断面回顾性研究。共收集了 104 例来自阿联酋北部地区 Thumbay 大学医院和其他医院妇科门诊 20-59 岁女性的液基宫颈细胞学样本，用于常规细胞学检查以识别和区分巴氏涂片样本的形态学变化。使用 HPV 直接流芯片法进行 HPV 基因分型。
结果
在 63 名（60.57%）阳性的女性中检测到 112 种 HPV 基因型，包括 18 例异常细胞学和 45 例正常上皮样本。在所有 63 例阳性样本中均检测到 63 种低危和 49 种高危 HPV 基因型。在 40-49 岁（25.9%）和 20-29 岁（23.5%）女性中，检测到多种低危和高危 HPV 基因型感染的发生率最高。感染 HPV6（13.46%）、HPV11（9.61%）、HPV16（9.61%）、HPV62/81（7.69%）和 HPV45（7.69%）是最常见的基因型。HPV45 和 62/81（7.69%）的检出率高于预期。20.2%的病例存在多种低危和高危基因型的混合感染；其中，HPV6（15.9%）最为常见，其次是 HPV62/81（12.7%）和 HPV16（11.11%）。HPV18 的检出率为 1.6%。
结论
6、45、16、11、67、62/81 型 HPV 是 21-59 岁女性中最常见的 HPV 感染类型。在研究人群中，HPV45、62/81 的检出率略有增加，HPV18 的检出率较低。43.27%的正常上皮样本对不同的低危和高危 HPV 基因型呈阳性。这一发现强调了 HPV 分子基因分型对宫颈筛查分类的重要性。
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