Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
Animal Production Research Institute, Agriculture Research Centre, Ministry of Agriculture, Dokki, Giza, 12619, Egypt.
Theriogenology. 2023 Dec;212:9-18. doi: 10.1016/j.theriogenology.2023.08.021. Epub 2023 Aug 30.
The existing treatise targeted to compare the effects of adding different nano-emulsions essential oils (olive, flaxseed, and grapeseed oils) in freezing extender on semen quality and freezability in buffalo. Nano-emulsions were prepared from olive, flaxseed, and grapeseed oils and characterized for their sizes and shapes. Semen extended in four tubes were supplemented with 0 (control) and 3.5% nanoemulsion oils, including olive (NEO), flaxseed (NEFO) and grape seed oils (NEGSO) respectively. NEGSO resulted in the highest (p < 0.05) membrane integrity, vitality, progressive motility (P-motility) of sperm compared to the other groups in post-thawed buffalo bull semen (at 37 °C for 30 s). The addition of NEGSO had the best results for membrane integrity, progressive motility, and vitality of sperm after incubation (at 37 °C and 5% CO for 2 h). A superior (p < 0.05) value of total antioxidant capacity in frozen-thawed spermatozoa was monitored in all supplemented groups as relative to the control. The values of malondialdehyde (MDA) and nitric oxide (NO) were lower (p < 0.05) in NEGSO group compared with other groups. Both NEO and NEFO exhibited the same results for MDA, and NO levels (p > 0.05). All supplemented groups exhibited lower hydrogen peroxide levels (p < 0.05) as relative to the un-treated group. The lowest (p < 0.05) caspase 3 levels were verified in NEGSO treatment, followed by NEFO and NEO treatments. Post-thawed sperm showed ultrastructural damages in the control group, and theses damages were attenuated or resorted by the NEGSO, NEFO and NEO supplemented to freezing extender. In consequences with in vitro results regarding the sperm attribute, a greater pregnancy rate (92%) was observed in NEGSO group as compared with NEFO (88%), NEO (76%) and CON (68%) groups. Our findings demonstrate that NEGSO (3.5%) could be used as a new strategy in enhancing sperm functionality, potential fertility and reducing the oxidative damage and apoptosis markers. This could be significantly applicable for sperm physiology cryopreservation in the milieu of assisted reproduction systems.
现有的论文旨在比较在冷冻液中添加不同纳米乳液（橄榄油、亚麻籽油和葡萄籽油）对水牛精液质量和可冻性的影响。纳米乳液由橄榄油、亚麻籽油和葡萄籽油制成，并对其大小和形状进行了特征描述。在四个试管中，将精液分别添加 0（对照）和 3.5%纳米乳液油，包括橄榄油（NEO）、亚麻籽油（NEFO）和葡萄籽油（NEGSO）。与其他组相比，解冻后的水牛公牛精液（在 37°C 下 30 秒）中，NEGSO 导致膜完整性、活力和精子的前向运动（P-运动）最高（p<0.05）。在孵育后（在 37°C 和 5%CO 下孵育 2 小时），添加 NEGSO 对精子的膜完整性、前向运动和活力具有最佳效果。与对照组相比，所有添加组的冷冻解冻精子的总抗氧化能力值均较高（p<0.05）。与其他组相比，NEGSO 组的丙二醛（MDA）和一氧化氮（NO）值较低（p<0.05）。NEO 和 NEFO 对 MDA 和 NO 水平的影响相同（p>0.05）。与未处理组相比，所有添加组的过氧化氢水平均较低（p<0.05）。NEGSO 处理组的半胱天冬酶 3 水平最低（p<0.05），其次是 NEFO 和 NEO 处理组。解冻后的精子在对照组中显示出超微结构损伤，而这些损伤在添加到冷冻液中的 NEGSO、NEFO 和 NEO 中得到减轻或恢复。根据体外精子属性的结果，NEGSO 组的妊娠率（92%）高于 NEFO 组（88%）、NEO 组（76%）和 CON 组（68%）。我们的研究结果表明，NEGSO（3.5%）可作为一种增强精子功能、潜在生育能力和降低氧化损伤和凋亡标志物的新策略。这在辅助生殖系统环境下的精子生理学冷冻保存中具有重要意义。
