Barenghi L, Ceriotti F, Luzzana M, Ripamonti M, Mosca A, Bonini P A
Ann Clin Biochem. 1986 Sep;23 ( Pt 5):538-45. doi: 10.1177/000456328602300509.
We describe a new electrochemical method for the determination of erythrocyte acetylcholinesterase activity (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) activity, based on the measurements of pH variation due to release of acetic acid from acetylcholine. The major advantages of the differential pH procedure are simplicity, high reproducibility, no need for pre-treatment of samples, automatic correction of sample blanks, and speed and direct measurement of enzymatic reaction. The proposed methods are linear up to 7400 U/L at 30 degrees C and correlate well with the manual spectrophotometric method of Ellman for plasma cholinesterase and for washed erythrocytes. We adapted the same technique for the determination of erythrocyte cholinesterase using whole blood as sample and quinidine sulphate as inhibitor of pseudocholinesterase.
我们描述了一种新的电化学方法，用于测定红细胞乙酰胆碱酯酶活性（EC 3.1.1.7）和血浆胆碱酯酶（EC 3.1.1.8）活性，该方法基于对乙酰胆碱释放乙酸导致的pH变化的测量。差分pH法的主要优点包括操作简单、重现性高、无需对样品进行预处理、能自动校正样品空白，以及酶促反应速度快且可直接测量。所提出的方法在30℃下线性范围可达7400 U/L，与用于血浆胆碱酯酶和洗涤红细胞的Ellman手动分光光度法相关性良好。我们采用相同技术，以全血为样品，硫酸奎尼丁为假性胆碱酯酶抑制剂，来测定红细胞胆碱酯酶。
