Lewis P J, Lowing R K, Gompertz D
Clin Chem. 1981 Jun;27(6):926-9.
We describe an automated kinetic method for erythrocyte acetylcholinesterase (EC 3.1.1.7) and plasma cholinesterase (EC 3.1.1.8) based on Ellman's colorimetric method. Quinidine sulfate is used as an inhibitor of plasma cholinesterase during the measurement of erythrocyte acetylcholinesterase activity, obviating the need for washing the erythrocytes before lysis. Results by this method are compared with those obtained by the electrometric delta pH method of Michel. To emphasize the need for measuring both erythrocyte acetylcholinesterase and plasma cholinesterase activity in workers exposed to organophosphate pesticides, we present a study of serial activities of both enzymes in a person accidentally exposed to demeton-S-methyl.
我们描述了一种基于埃尔曼比色法的红细胞乙酰胆碱酯酶（EC 3.1.1.7）和血浆胆碱酯酶（EC 3.1.1.8）的自动动力学方法。在测定红细胞乙酰胆碱酯酶活性期间，使用硫酸奎尼丁作为血浆胆碱酯酶的抑制剂，从而无需在红细胞裂解前进行洗涤。将该方法得到的结果与通过米歇尔的电位差pH法获得的结果进行比较。为强调在接触有机磷农药的工人中同时测量红细胞乙酰胆碱酯酶和血浆胆碱酯酶活性的必要性，我们展示了一项针对一名意外接触甲基内吸磷的人员的这两种酶的系列活性研究。
