Department of Food Chemistry & Technology, Teagasc Food Research Centre, Ashtown, Ireland; School of Chemical and Bio Pharmaceutical Sciences, Technological University Dublin, Ireland; Department of Biological Sciences, Munster Technological University, Bishopstown, Cork T12P928, Ireland.
Department of Applied Science, Technological University of Shannon: Midlands and Midwest, Moylish, Co Limerick, Ireland.
Food Chem. 2024 Feb 1;433:137259. doi: 10.1016/j.foodchem.2023.137259. Epub 2023 Aug 25.
This study investigated the multiproduct (fucoidans, β-glucans, proteins, carotenoids, fatty acids, amino acids and polyphenols) valorization of the invasive macroalgae Ascophyllum nodosum within a green biorefinery concept using ultrasound (US), microwave (MW) treatment followed by supercritical CO (SC-CO) with co-solvent and enzymatic extraction. Water and 50% aqueous ethanol were used as green extraction solvents. The extraction methods using 50% ethanol as extraction solvent improved the yields of phenolic compounds and glucan and enhanced in vitro antioxidant activity. The characterization of SC-CO extracts revealed that pretreatment with US and MW improved the 2-fold yield of carotenoids, total phenolics and fatty acids. However, US/MW pretreatment and enzymatic extraction did not improve the yields of proteins and free amino acids. Overall, using concurrent green US/MW-assisted extraction methods enhanced the yields of the bioactive compounds in a short duration and provided extracts with a better antioxidant capacity in the field of food applications.
本研究在绿色生物炼制概念下，利用超声（US）、微波（MW）处理后结合超临界 CO（SC-CO）与共溶剂和酶提取，研究了入侵大型海藻泡叶藻的多产物（褐藻糖胶、β-葡聚糖、蛋白质、类胡萝卜素、脂肪酸、氨基酸和多酚）增值。水和 50%的乙醇水溶液被用作绿色提取溶剂。使用 50%乙醇作为提取溶剂的提取方法提高了酚类化合物和葡聚糖的产率，并增强了体外抗氧化活性。SC-CO 提取物的特性表明，US 和 MW 的预处理提高了类胡萝卜素、总酚类和脂肪酸的 2 倍产率。然而，US/MW 预处理和酶提取并没有提高蛋白质和游离氨基酸的产率。总的来说，同时使用绿色 US/MW 辅助提取方法可以在短时间内提高生物活性化合物的产率，并在食品应用领域提供具有更好抗氧化能力的提取物。
