Symrise Srl, Padova, Italy.
Clinic of Plastic and Reconstructive Surgery, University of Padova, Padova, Italy.
Photochem Photobiol. 2024 May-Jun;100(3):733-745. doi: 10.1111/php.13850. Epub 2023 Sep 7.
Sunlight, and more specifically the UV component, induces several skin damages, including sunburns, erythema and photoaging. The purpose of this work is to set up an ex vivo human skin model to assess the capacity of active principles in protecting skin from the deleterious effects of solar radiation. Ex vivo human skin biopsies were cultured in an air-liquid interface and exposed to solar-simulated radiation (SSR, 300-750 nm). L-Carnosine (0.2% and 2%) was applied topically to be tested as photoprotective compound. The effect on oxidative stress induction, photoaging and skin transcriptional profile was assessed by evaluating reactive oxygen species, advanced glycosylation end products formation and gene expression changes. In our model, SSR increases ROS production and AGE accumulation and affects the expression of genes related to oxidative stress, pigmentation, immunity, inflammation and photoaging. Among these pathways, 11 genes were selected as biomarkers to evaluate the skin solar radiation response. Results showed that L-Carnosine provides effective prevention against solar radiation damages reducing ROS, AGEs and mitigating the modulation of the selected biomarker genes. In conclusion, we report that our ex vivo skin model is a valuable system to assess the consequences of solar light exposure and the capacity of topically applied L-Carnosine to counteract them.
阳光，特别是紫外线成分，会导致多种皮肤损伤，包括晒伤、红斑和光老化。本工作的目的是建立一个离体人皮肤模型，以评估活性成分保护皮肤免受太阳辐射有害影响的能力。离体人皮肤活检标本在气-液界面中培养，并暴露于模拟太阳辐射（SSR，300-750nm）下。将 L-肉碱（0.2%和 2%）局部应用以测试其作为光保护化合物的效果。通过评估活性氧、晚期糖基化终产物形成和基因表达变化来评估其对氧化应激诱导、光老化和皮肤转录谱的影响。在我们的模型中，SSR 增加了 ROS 的产生和 AGE 的积累，并影响了与氧化应激、色素沉着、免疫、炎症和光老化相关的基因表达。在这些途径中，选择了 11 个基因作为生物标志物来评估皮肤对太阳辐射的反应。结果表明，L-肉碱能有效预防太阳辐射损伤，减少 ROS、AGEs，并减轻所选生物标志物基因的调节。总之，我们报告说，我们的离体皮肤模型是评估太阳光照暴露后果的有价值系统，以及局部应用 L-肉碱对抗这些后果的能力。
