Li Yuan, Wang Zhanhui
National Key Laboratory of Veterinary Public Health Security, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People's Republic of China.
Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou 311300, People's Republic of China.
ACS Appl Mater Interfaces. 2023 Sep 20;15(37):43223-43225. doi: 10.1021/acsami.3c08653. Epub 2023 Sep 7.
Listek et al. described some technical concerns for the single transgenic hybridoma selection method (STHSM) that we developed. In this work, we developed the STHSM to screen hapten-specific hybridoma cells based on a streptavidin-labeled antigen originally proposed by Listek et al. that was used for the selection of OVA-specific hybridoma. Due to the obvious differences between hapten with a single epitope and protein with multiple epitopes, we significantly innovated the specific procedures in the STHSM that could answer the questions raised by Listek's group.
利斯特克等人描述了我们开发的单转基因杂交瘤选择方法（STHSM）的一些技术问题。在这项工作中，我们开发了STHSM，以基于利斯特克等人最初提出的链霉亲和素标记抗原筛选半抗原特异性杂交瘤细胞，该抗原用于选择OVA特异性杂交瘤。由于具有单个表位的半抗原与具有多个表位的蛋白质之间存在明显差异，我们在STHSM中显著创新了特定程序，以回答利斯特克团队提出的问题。
