Immunotechnology Group, Institute of Biochemistry and Biology, University of Potsdam, 14469 Potsdam, Germany.
new/era/mabs GmbH, August-Bebel-Str. 89, 14482 Potsdam, Germany.
ACS Appl Mater Interfaces. 2023 Sep 20;15(37):43219-43222. doi: 10.1021/acsami.3c05317. Epub 2023 Sep 7.
In the original paper, Li and co-workers [ , , 17128-17141] described their approach to select specific hybridoma cells from a polyclonal hybridoma pool by using a cell surface anchor to catch the secreted antibody. The antigen-specific detection was performed with streptavidin-labeled antigen and a PE-labeled anti-F(ab') antibody. The present comment offers a clearer description of the selection system originally published by Listek et al. in 2020 and provides further information about the importance of controls and recent adaptations made by our lab.
在原始论文中，Li 及其同事 [,, 17128-17141] 描述了他们通过使用细胞表面锚定物捕获分泌的抗体，从多克隆杂交瘤池中选择特定杂交瘤细胞的方法。通过链霉亲和素标记的抗原和 PE 标记的抗 F(ab') 抗体进行抗原特异性检测。本评论更清晰地描述了 Listek 等人在 2020 年发表的选择系统，并提供了有关对照的重要性和我们实验室最近的改进的更多信息。
