College of Acupuncture and Tuina and Rehabilitation, Hunan University of Chinese Medicine.
College of Acupuncture and Tuina and Rehabilitation, Hunan University of Chinese Medicine;
J Vis Exp. 2023 Aug 25(198). doi: 10.3791/65820.
Ding's roll method is one of the most commonly used manipulations in traditional Chinese massage (Tuina) clinics and one of the most influential contemporary Tuina manipulations in China. It is based on the traditional rolling method commonly used in the one finger Zen genre and named Ding's roll method. Due to its anti-inflammatory and blood circulation-promoting effects, Ding's rolling method has sound therapeutic effects on myopathy. Because of the large area of force applied to human skin, Ding's roll method is challenging to perform on experimental animals with small skin areas, such as rats and rabbits. Additionally, the strength of Tuina applied to the human body differs from that applied to experimental animals, so it may happen that the strength is too high or too low to achieve the therapeutic effect of Tuina during the experiment. This experiment aims to create a simple massager suitable for rats based on Ding's rolling manipulation parameters (strength, frequency, Tuina duration). The device can standardize manipulation in animal experiments and reduce the variation in Tuina force applied to different animals due to subjective factors. A rat model of notexin-induced skeletal muscle injury was established, and plasma injury markers creatine kinase (CK) and fatty acid binding protein 3 (FABP3) were used to assess the therapeutic effect of Tuina on skeletal muscle injury. The results showed that this Tuina massager could reduce the levels of CK and FABP3 expression and slow down the degree of skeletal muscle injury. Therefore, the Tuina massager described here, mimicking Ding's roll method, contributes to standardizing Tuina manipulation in experimental research and is of great help for subsequent research on the molecular mechanism of Tuina for myopathy.
丁氏滚法是传统中医推拿（推拿）诊所中最常用的手法之一，也是中国当代最有影响力的推拿手法之一。它是基于一指禅流派中常用的传统滚法，并以丁氏命名。由于其具有抗炎和促进血液循环的作用，丁氏滚法对肌病有很好的治疗效果。由于该方法在人皮肤上施加的力面积较大，因此对于皮肤面积较小的实验动物（如大鼠和兔子）来说，丁氏滚法的操作具有一定的挑战性。此外，推拿施加到人体上的力与施加到实验动物上的力不同，因此在实验过程中可能会出现力过高或过低，无法达到推拿的治疗效果。本实验旨在根据丁氏滚法的操作参数（强度、频率、推拿持续时间），为大鼠制作一种简单的按摩器。该装置可以使动物实验中的操作标准化，并减少由于主观因素导致的不同动物之间推拿力的差异。建立了蓖麻毒素诱导的骨骼肌损伤大鼠模型，使用血浆损伤标志物肌酸激酶（CK）和脂肪酸结合蛋白 3（FABP3）评估推拿对骨骼肌损伤的治疗效果。结果表明，这种推拿按摩器可以降低 CK 和 FABP3 表达水平，减缓骨骼肌损伤程度。因此，这里描述的模仿丁氏滚法的推拿按摩器有助于规范实验研究中的推拿操作，对后续研究推拿治疗肌病的分子机制有很大帮助。
