使用单克隆抗-N抗体进行血型鉴定。

Blood grouping with monoclonal anti-N antibodies.

作者信息

Fletcher A, Harbour C, Matthews M, de Zwart R, Ford D

出版信息

Aust J Exp Biol Med Sci. 1986 Jun;64 ( Pt 3):215-22. doi: 10.1038/icb.1986.23.

DOI:10.1038/icb.1986.23

Abstract

Four monoclonal antibodies (N1, N3, N4 and N5) with specificity for the N blood group have been produced from fusions in which human erythrocytes were used as the immunizing stimulus. N1 and N3 differed from N4 and N5 with regard to their serological specificities and pH optima. Antibody N3 has been assessed for its suitability as a blood grouping reagent by testing 10,767 blood donors in parallel with Vicia graminea anti-N lectin or rabbit anti-N. There was complete correlation between the results obtained with the monoclonal and conventional reagents providing strong evidence that N3 is a reliable and potent blood grouping reagent.

摘要

利用人红细胞作为免疫刺激物进行细胞融合，已产生了4种对N血型具有特异性的单克隆抗体（N1、N3、N4和N5）。N1和N3在血清学特异性和最适pH值方面与N4和N5不同。通过与蚕豆抗N凝集素或兔抗N抗体同时检测10767名献血者，评估了抗体N3作为血型鉴定试剂的适用性。单克隆试剂和传统试剂所得结果完全相关，有力证明N3是一种可靠且有效的血型鉴定试剂。