Blood grouping with monoclonal antibodies.

The Technicon BG15 automated blood grouping machine has been used to type over 32,000 blood donors with a monoclonal anti-A and anti-B. The results of using these antibodies in parallel with conventional human antisera will be presented along with preliminary data obtained with the Groupamatic 2000 using a different anti-A. A monoclonal anti-N reagent has been assessed for its suitability for use as a blood grouping reagent in microplate assays by testing 10,000 blood donors in parallel with Vicia graminea anti-N lectin or rabbit anti-N. There was complete correlation between the results obtained with the monoclonal antibodies and conventional reagents.