Lin Yuzhen, Xin Zhiming, Xia Yuhong, Wen Xinan, Xin Fan, Ruan Guohong
Public Health School of Fujian Medical University, Fuzhou 350122, China.
Fujian Center for Safety Evaluation of New Drug, Fuzhou 350122, China.
Wei Sheng Yan Jiu. 2023 Jul;52(4):598-603. doi: 10.19813/j.cnki.weishengyanjiu.2023.04.013.
To explore the effect of strontium in drinking water on blood pressure in hypertensive mice and its possible mechanism.
Establishment of mouse model of high blood pressure by drinking 2 mg/mL N'nitro-L-arginine methyl eater hydrochloride(L-NAME) for 4 weeks. One hundred ICR mice were randomly divided into normal control group(n=20) and model group(n=80) according to systolic blood pressure. Eighty hypertensive mice were randomly divided into model control group(n=20), 2.5 mg/L strontium water group(n=20), 5.0 mg/L strontium water group(n=20) and 10.0 mg/L strontium water group(n=20). The body weight and blood pressure of mice were measured every week. After 10 weeks, serum sodium(Na), potassium(K), calcium(Ca), magnesium(Mg), Chlorine(Cl), nitric oxide(NO), renin, angiotensin II(Ang II), aldosterone(ALD), endothelial nitric oxide synthase(eNOS), intercellular cell adhesion molecule-1(ICAM-1), heart interleukin-6(IL-6), interleukin-1 beta(IL-1β) and tumor necrosis factor-α(TNF-α) were determined.
After 10 weeks of intervention, the systolic blood pressure in the low, medium and high strontium water groups(129.60±4.90 mmHg vs.127.33±6.35 mmHg vs.124.70±3.91 mmHg) was significantly lower than that of the model control group(141.84±5.34 mmHg)(P<0.05). The diastolic blood pressure in the high strontium water group(84.74±5.49 mmHg) was significantly lower than that of the model control group(92.21±10.08 mmHg). The contents of serum potassium, calcium and magnesium in medium strontium gourp(8.06±0.80 mmol/L vs.2.34±0.13 mmol/L vs.0.57±0.12 mmol/L) and high strontium group(9.59±0.58 mmol/L vs. 2.37±0.17 mmol/L vs.0.58±0.09 mmol/L) were significantly higher than those in normal control group(6.64±0.57 mmol/L vs.2.07±0.15 mmol/L vs.0.46±0.10 mmol/L) and model control group(6.62±0.53 mmol/L vs.2.09±0.11 mmol/L vs.0.48±0.09 mmol/L)(P<0.05). Compared with model control group, the contents of renin(24.08±6.65 ng/mL vs.15.24±3.88 ng/mL), AngII(263.30±61.66 pg/mL vs.203.31±54.95 pg/mL), ALD(102.41±22.39 pg/mL vs. 60.31±10.83 pg/mL), ICAM-1(367.17±120.08 ng/mL vs.224.45±46.86 ng/mL), IL-6(5.90±0.66 ng/mL vs.3.88±1.08 ng/mL), IL-1β(6.37±1.83 ng/mL vs.3.44±1.28 ng/mL) and TNF-α(9.35±1.41 ng/mL vs.5.68±2.11 ng/mL) in high strontium group were significantly decreased(P<0.05).
Strontium can reduce the blood pressure of hypertensive mice by regulating the eNOS/NO pathway and reducing the production of inflammatory factors.
探讨饮用水中锶对高血压小鼠血压的影响及其可能机制。
通过饮用2 mg/mL盐酸N'-硝基-L-精氨酸甲酯（L-NAME）4周建立小鼠高血压模型。将100只ICR小鼠按收缩压随机分为正常对照组（n = 20）和模型组（n = 80）。80只高血压小鼠随机分为模型对照组（n = 20）、2.5 mg/L锶水组（n = 20）、5.0 mg/L锶水组（n = 20）和10.0 mg/L锶水组（n = 20）。每周测量小鼠体重和血压。10周后，测定血清钠（Na）、钾（K）、钙（Ca）、镁（Mg）、氯（Cl）、一氧化氮（NO）、肾素、血管紧张素II（Ang II）、醛固酮（ALD）、内皮型一氧化氮合酶（eNOS）、细胞间黏附分子-1（ICAM-1）、心脏白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）。
干预10周后，低、中、高锶水组的收缩压（129.60±4.90 mmHg对127.33±6.35 mmHg对124.70±3.91 mmHg）显著低于模型对照组（141.84±5.34 mmHg）（P<0.05）。高锶水组的舒张压（84.74±5.49 mmHg）显著低于模型对照组（92.21±10.08 mmHg）。中锶组（8.06±0.80 mmol/L对2.34±0.13 mmol/L对0.57±0.12 mmol/L）和高锶组（9.59±0.58 mmol/L对2.37±0.17 mmol/L对0.58±0.09 mmol/L）血清钾、钙和镁的含量显著高于正常对照组（6.64±0.57 mmol/L对2.07±0.15 mmol/L对0.46±0.10 mmol/L）和模型对照组（6.62±0.53 mmol/L对2.09±0.11 mmol/L对0.48±0.09 mmol/L）（P<0.05）。与模型对照组相比，高锶组肾素（24.08±6.65 ng/mL对15.24±3.88 ng/mL）、AngII（263.30±61.66 pg/mL对203.31±54.95 pg/mL）、ALD（102.41±22.39 pg/mL对60.31±10.83 pg/mL）、ICAM-1（367.17±120.08 ng/mL对224.45±46.86 ng/mL）、IL-6（5.90±0.66 ng/mL对3.88±1.08 ng/mL）、IL-1β（6.37±1.83 ng/mL对3.44±1.28 ng/mL）和TNF-α（9.35±1.41 ng/mL对5.68±2.11 ng/mL）的含量显著降低（P<0.05）。
锶可通过调节eNOS/NO途径并减少炎症因子的产生来降低高血压小鼠的血压。
