Sun Qian, Chen Ronghe, Xin Fan, Ruan Guohong
Department of Health Inspection and Quarantine, Fujian Medical University, Fuzhou 350108, China.
Wei Sheng Yan Jiu. 2017 Nov;46(6):899-904.
To explore the effect of three kinds of drinking water and its possible mechanism on blood pressure of hypertensive mice.
The water quality parameters were measured for three kinds of drinking water, and the parameters includedtotal dissolved solids( TDS), oxygen consumed( OC), p H, oxidation reduction potential( ORP), electric conductivity( EC), dissolved hydrogen, calcium and magnesium. Establishment of mouse model of high blood pressure by using N'-nitro-L-arginine methyl ester hydrochloride( L-NAME) for 30 days. Then the mice were randomly divided into 4 groups: normal control group, model control group, group of tap water and group of filtered water. Blood pressure in mice was detected every month. After 3 months, nitric oxide( NO), nitric oxide synthase( NOS), total superoxide dismutase( T-SOD), malondialdehyde( MDA), glutathione peroxidase( GSH-Px), endothelin( ET), angiotensin( Ang), aldosterone( ALD), catecholamine( CA), high-sensitivity C-reactive protein( hs-CRP), interleukin 6( IL-6) and cyclic guanosine monophosphate( c-GMP) in serum of the mice were determined. Simultaneously morphological changes of heart, kidney and thoracic aorta paraffin section were observed.
The p H, TDS, OC, EC, calcium and magnesium in filtered water were higher than that of pure and tap water. The negative ORP and great quantities of dissolved hydrogen were found in filtered water; The systolic blood pressure and mean blood pressure(( 106. 24 ± 5. 31) and( 90. 73 ± 4. 99)mm Hg) of filtered water were lower than those of the pure water( 119. 58 ± 6. 08 and 96. 44± 6. 48 mm Hg)( P < 0. 05). The NO(( 87. 05 ± 39. 82) μmol/L) in the mice with filtered water were higher than those of with pure and tap water(( 45. 01 ± 9. 62) and( 46. 56 ±30. 54) μmol/L)( P < 0. 05). Compared with those of the mice with pure water, significant decrease in IL-6(( 201. 42 ± 36. 41) and( 173. 99 ± 114. 96) vs. ( 363. 14 ± 149. 00)pg/m L) and Ang(( 1319. 20 ± 111. 90) and( 1349. 38 ± 180. 15) vs. ( 1736. 17 ±242. 86) ng/L) were observed in mice treated with tap and filtered water( P < 0. 05). The T-SOD and GSH-Px(( 268. 37 ± 12. 25) and( 712. 45 ± 30. 59) U/m L) in the mice with filtered water were significant higher than those of pure water(( 250. 46 ± 15. 60) and( 678. 36 ± 35. 80) U/m L)( P < 0. 05).
Compared with pure and tap water, filtered water can reduce the blood pressure of the hypertensive mice by antioxidation and anti-inflammatory.
探讨三种饮用水对高血压小鼠血压的影响及其可能机制。
测定三种饮用水的水质参数，包括总溶解固体（TDS）、耗氧量（OC）、pH值、氧化还原电位（ORP）、电导率（EC）、溶解氢、钙和镁。用盐酸N'-硝基-L-精氨酸甲酯（L-NAME）建立高血压小鼠模型30天。然后将小鼠随机分为4组：正常对照组、模型对照组、自来水组和过滤水组。每月检测小鼠血压。3个月后，测定小鼠血清中一氧化氮（NO）、一氧化氮合酶（NOS）、总超氧化物歧化酶（T-SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、内皮素（ET）、血管紧张素（Ang）、醛固酮（ALD）、儿茶酚胺（CA）、高敏C反应蛋白（hs-CRP）、白细胞介素6（IL-6）和环磷酸鸟苷（c-GMP）。同时观察心脏、肾脏和胸主动脉石蜡切片的形态学变化。
过滤水中的pH值、TDS、OC、EC、钙和镁高于纯净水和自来水。过滤水中发现负的ORP和大量溶解氢；过滤水组小鼠的收缩压和平均血压（（106.24±5.31）和（90.73±4.99）mmHg）低于纯净水组（119.58±6.08和96.44±6.48 mmHg）（P<0.05）。过滤水组小鼠的NO（（87.05±39.82）μmol/L）高于纯净水和自来水组（（45.01±9.62）和（46.56±30.54）μmol/L）（P<0.05）。与纯净水组小鼠相比，自来水组和过滤水组小鼠的IL-6（（201.42±36.41）和（173.99±114.96）对（363.14±149.00）pg/mL）和Ang（（1319.20±111.90）和（1349.38±180.15）对（1736.17±242.86）ng/L）显著降低（P<0.05）。过滤水组小鼠的T-SOD和GSH-Px（（268.37±12.25）和（712.45±30.59）U/mL）显著高于纯净水组（（250.46±15.60）和（678.36±35.80）U/mL）（P<0.05）。
与纯净水和自来水相比，过滤水可通过抗氧化和抗炎作用降低高血压小鼠的血压。
