ARC Training Centre for Innovative Horticultural Products, Tasmania Institute of Agriculture, University of Tasmania, Private Bag 54, Hobart, TAS 7001, Australia.
Tasmania Institute of Agriculture, University of Tasmania, Private Bag 54, Hobart, TAS 7001, Australia.
Lett Appl Microbiol. 2023 Sep 1;76(9). doi: 10.1093/lambio/ovad104.
Resin canal discoloration (RCD) severely impacts the fruit quality of mango, diminishes consumer confidence, and reduces sales, but the biological cause is still unclear. Using next-generation sequencing, the overall microbial community composition of RCD+ and visually healthy mango fruits was determined for the first time to examine the possible role of bacterial and fungal pathogens in RCD. The diversity profile of bacterial and fungal communities was determined using primers targeting the 16S rRNA gene and Internal Transcribed Spacer (ITS) regions. Results showed that bacterial communities in healthy fruits are clustered together and significantly different from those in RCD+ fruits. Tatumella and Pantoea species were the most abundant bacterial taxa on RCD+ fruit, and both have been linked to disease outbreaks in a variety of fruit crops. Fungal communities were generally similar between RCD+ and normal samples, though non-pathogenic yeasts Meyerozyma and Naganishia tended to dominate the fungal communities on RCD+ fruit. The study indicates that bacteria rather than fungal organisms are more likely to be associated with RCD in mango. This finding will facilitate the isolation and confirmation of RCD-causing organisms and the development of control strategies to manage RCD problem in mango.
树脂道变色（RCD）严重影响芒果的果实品质，降低消费者信心，减少销售，但生物原因仍不清楚。本研究首次使用下一代测序技术，确定了 RCD+和视觉健康芒果果实的整体微生物群落组成，以检查细菌和真菌病原体在 RCD 中的可能作用。使用针对 16S rRNA 基因和内部转录间隔区（ITS）区域的引物来确定细菌和真菌群落的多样性图谱。结果表明，健康果实中的细菌群落聚集在一起，与 RCD+果实中的细菌群落有显著差异。Tatumella 和 Pantoea 属是 RCD+果实上最丰富的细菌分类群，两者都与多种水果作物的疾病爆发有关。真菌群落通常在 RCD+和正常样本之间相似，尽管非致病性酵母 Meyerozyma 和 Naganishia 倾向于在 RCD+果实上占主导地位。该研究表明，与 RCD 相关的更可能是细菌而不是真菌。这一发现将有助于分离和确认 RCD 致病生物，并制定控制策略来管理芒果的 RCD 问题。
