Boyd W H, Krogsrud R, Jichici D
Biochem Biophys Res Commun. 1986 Sep 14;139(2):480-6. doi: 10.1016/s0006-291x(86)80016-x.
Met-enkephalin, and opioid peptide present in the intermediate lobe of the pituitary gland, is herein demonstrated, for the first time, in bovine pituitary intraglandular colloid. Colloid samples were taken from the glands of freshly slaughtered cattle, pooled, lyophilized, extracted on octadecylsilyl-silica and assessed by radioimmunoassay. Met-enkephalin was indeed present in this colloid extract at levels varying from 0.40 to 0.85 nanograms per milligram dry weight. Locating an intermediate lobe peptide in intraglandular colloid is a significant finding since this implies that colloid, housed in the intraglandular lumen, may serve as a transport medium. Intermediate lobe peptides which, because the intermediate lobe is avascular, are otherwise denied the vascular transport routes available to the anterior and posterior lobe hormones.
甲硫氨酸脑啡肽，一种存在于垂体中间叶的阿片样肽，首次在牛垂体腺内胶体中得到证实。从刚屠宰的牛的腺体中采集胶体样本，合并、冻干，在十八烷基硅烷硅胶上进行提取，并通过放射免疫测定法进行评估。在这种胶体提取物中确实存在甲硫氨酸脑啡肽，其含量为每毫克干重0.40至0.85纳克不等。在腺内胶体中定位中间叶肽是一项重要发现，因为这意味着存在于腺内腔中的胶体可能充当运输介质。由于中间叶无血管，中间叶肽无法通过前叶和后叶激素所利用的血管运输途径。
