聚（ADP - 核糖）能有效地与DNA竞争结合组蛋白H4。
Poly(ADP-ribose) effectively competes with DNA for histone H4 binding.
作者信息
Sauermann G, Wesierska-Gadek J
出版信息
Biochem Biophys Res Commun. 1986 Sep 14;139(2):523-9. doi: 10.1016/s0006-291x(86)80022-5.
The effect of poly(ADP-ribose) on DNA-histone H4 interaction was studied using a nitrocellulose filter binding assay. Poly-(ADP-ribose) was found to form poly(ADP-ribose)-histone H4 complexes at physiological salt concentrations. The homopolymer effectively competed with DNA for histone H4 binding. Poly(ADP-ribose) was also capable of displacing DNA from preformed DNA-histone H4 complexes. Our hypothesis is that poly(ADP-ribose), locally and transiently formed at the site of DNA damage, causes dissociation of DNA from the nucleosome particle or nucleosome unfolding.
使用硝酸纤维素滤膜结合试验研究了聚（ADP - 核糖）对DNA - 组蛋白H4相互作用的影响。发现在生理盐浓度下，聚（ADP - 核糖）会形成聚（ADP - 核糖）-组蛋白H4复合物。该均聚物能有效竞争DNA与组蛋白H4的结合。聚（ADP - 核糖）还能够将DNA从预先形成的DNA - 组蛋白H4复合物中置换出来。我们的假设是，在DNA损伤部位局部和瞬时形成的聚（ADP - 核糖）会导致DNA从核小体颗粒上解离或核小体解折叠。
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