George C X, Atwater J A, Samuel C E
Biochem Biophys Res Commun. 1986 Sep 14;139(2):845-51. doi: 10.1016/s0006-291x(86)80067-5.
Human reovirus serotype 1 Lang strain s3 mRNA, which encodes the nonstructural RNA-binding polypeptide sigma NS, was cloned as a cDNA:mRNA heteroduplex in Escherichia coli using phage M13. A complete consensus nucleotide sequence was determined. The Lang strain s3 mRNA is 1198 nucleotides in length and possesses an open reading frame with a coding capacity of 366 amino acids, sufficient to account for a sigma NS polypeptide of 41,179 daltons. Comparison of the serotype 1 (Lang) s3 sequence with the serotype 3 (Dearing) s3 sequence reveals 86.8 percent homology at the nucleotide level. The predicted sigma NS polypeptides of the Lang and Dearing strains display 97 percent homology at the amino acid level.
人呼肠孤病毒1型朗株s3 mRNA编码非结构RNA结合多肽σNS，利用噬菌体M13在大肠杆菌中作为cDNA:mRNA异源双链体进行克隆。确定了完整的共有核苷酸序列。朗株s3 mRNA长度为1198个核苷酸，具有一个开放阅读框，编码能力为366个氨基酸，足以编码一个41,179道尔顿的σNS多肽。1型（朗株）s3序列与3型（迪林株）s3序列在核苷酸水平上的同源性为86.8%。朗株和迪林株预测的σNS多肽在氨基酸水平上的同源性为97%。
