Cashdollar L W, Esparza J, Hudson G R, Chmelo R, Lee P W, Joklik W K
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7644-8. doi: 10.1073/pnas.79.24.7644.
The genes of the Dearing strain of reovirus serotype 3, which consist of double-stranded RNA, have been cloned into pBR322 by tailing both strands of each gene with poly(A), transcribing them with reverse transcriptase, self-hybridizing the cognate plus and minus cDNA strands, incubating them with Escherichia coli DNA polymerase I to ensure that they are complete, and cloning the double-stranded cDNA molecules by standard procedures. The sequence of the cloned S2 gene has been determined. The sequence at the termini are exactly the same as those at the ends of the native double-stranded RNA gene. The gene is 1,329 nucleotides long and possesses a single long open reading frame that starts at the first initiation codon (residue 19) and extends for 331 codons, sufficient to encode a protein of the same size as the known S2 gene product, protein sigma 2, a major reovirus core component (Mr, 38,000). A second open reading frame of 85 codons, in a different phase, starts close to where the first ends. The protein translated from this reading frame is unknown.
呼肠孤病毒3型迪尔林毒株的基因由双链RNA组成，通过对每个基因的两条链都加上聚腺苷酸尾，用逆转录酶进行转录，使同源的正链和负链cDNA自杂交，与大肠杆菌DNA聚合酶I一起孵育以确保其完整性，然后通过标准程序克隆双链cDNA分子，从而将其克隆到pBR322中。已确定克隆的S2基因的序列。其末端序列与天然双链RNA基因末端的序列完全相同。该基因长1329个核苷酸，具有一个单一的长开放阅读框，从第一个起始密码子（第19位残基）开始，延伸331个密码子，足以编码一个与已知S2基因产物大小相同的蛋白质，即蛋白质σ2，一种主要的呼肠孤病毒核心成分（分子量为38000）。另一个有85个密码子的开放阅读框处于不同相位，起始位置靠近第一个开放阅读框的末端。从这个阅读框翻译出的蛋白质尚不清楚。
