Department of Physiology, Anhui Medical College, Hefei 230601, China.
Department of Physiology, Wannan Medical College, Wuhu 241002, China.
J Tradit Chin Med. 2023 Oct;43(5):925-933. doi: 10.19852/j.cnki.jtcm.20230727.002.
To investigate the outcome of Chinese water-soluble propolis (WSP) on the inflammatory response and oxidative stress (OS) of colonic mucosa in rats with ulcerative colitis.
Dextran sulfate sodium (DSS) was employed to establish the ucerative colitis (UC) rat model. Forty-eight male rats were arbitrarily separated into six groups, namely control, UC, low-dose water-soluble propolis (L-WSP), medium-dose water-soluble propolis (M-WSP), high-dose water-soluble propolis (H-WSP), and sulfasalazine (Sulfa). In this study, we adopted a method of pre-administration and reconstruction of the model that assessed the water-soluble propolis mediated protection against DSS-induced UC rats. Moreover, we examined the body weight (BW), disease activity index (DAI), bloody stool, colon length, and intestinal mucosal injury index of rats. In addition, using enzyme linked immunosorbent assays, we assessed indicators, such as, colonic myeloperoxidase (MPO), interleukin-6 (IL-6), interleukin-9 (IL-9), tumor necrosis factor-ɑ (TNF-ɑ), superoxide dismutase (SOD), malondialdehyde, and glutathione peroxidase (GSH-Px) levels.
The pro-inflammatory cytokine expression, as well as OS, was increased in the model rats. However, upon WSP intervention, both pro-inflammatory cytokine levels and OS reduced dramatically, and the therapeutic effect was dose-dependent.
WSP downregulates OS by enhancing the function of endogenous antioxidant enzymes like SOD and GSH-Px, that inhibit neutrophil activity, as well as diminish pro-inflammatory cytokines like TNF-ɑ, IL-6, and IL-9, along with mechanisms that attenuate intestinal inflammation in UC rat model.
研究中国水溶性蜂胶（WSP）对溃疡性结肠炎大鼠结肠黏膜炎症反应和氧化应激（OS）的影响。
采用葡聚糖硫酸钠（DSS）建立溃疡性结肠炎（UC）大鼠模型。将 48 只雄性大鼠随机分为 6 组，分别为对照组、UC 组、低剂量水溶性蜂胶（L-WSP）组、中剂量水溶性蜂胶（M-WSP）组、高剂量水溶性蜂胶（H-WSP）组和柳氮磺胺吡啶（Sulfa）组。本研究采用模型预给药和重建的方法，评估水溶性蜂胶对 DSS 诱导的 UC 大鼠的保护作用。检测大鼠的体重（BW）、疾病活动指数（DAI）、血便、结肠长度和肠黏膜损伤指数。此外，采用酶联免疫吸附试验检测大鼠结肠髓过氧化物酶（MPO）、白细胞介素-6（IL-6）、白细胞介素-9（IL-9）、肿瘤坏死因子-ɑ（TNF-ɑ）、超氧化物歧化酶（SOD）、丙二醛和谷胱甘肽过氧化物酶（GSH-Px）水平等指标。
模型大鼠促炎细胞因子表达及 OS 增加，WSP 干预后促炎细胞因子水平及 OS 显著降低，且呈剂量依赖性。
WSP 通过增强 SOD 和 GSH-Px 等内源性抗氧化酶的功能，抑制中性粒细胞活性，降低 TNF-ɑ、IL-6 和 IL-9 等促炎细胞因子，减轻 UC 大鼠模型的肠道炎症，从而下调 OS。
