Balaha Mohamed, Kandeel Samah, Elwan Walaa
Pharmacology Department, Faculty of Medicine, Tanta University, Postal No. 31527, El-Gish Street, Tanta, Egypt.
Histology Department, Faculty of Medicine, Tanta University, Postal No. 31527, El-Gish Street, Tanta, Egypt.
Life Sci. 2016 Feb 1;146:40-51. doi: 10.1016/j.lfs.2016.01.012. Epub 2016 Jan 11.
Garlic oil (GO) is used for centuries in folk medicine as a therapy for many diseases including inflammatory disorders. Recently, it has exhibited potent anti-oxidant, anti-inflammatory and immunomodulatory effects. Consequently, we evaluated the possible protective effect of GO in a rat model of colitis, induced by dextran sulfate sodium (DSS).
Colitis induced by allowing rats a free access to drinking water containing 5% DSS for 7 days, from day 1 to day 7. GO was administered orally in doses of 25, 50 and 100mg/kg/day. Mesalazine used as a standard medication in a dose of 15 mg/kg/day. All animals fasted for 2h, 1h before and 1h after giving the treatment, which introduced daily for 7 days, from day 1 to day 7, at 10:00 to 11:00 A.M. Animal body, and colonic weights, colonic myeloperoxidase (MPO), and superoxide dismutase (SOD) activities, colonic reduced-glutathione (GSH), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-10 levels, macroscopic and microscopic changes of colonic tissues were evaluated.
GO treatment significantly suppressed the elevated colonic weight, MPO activity, MDA, TNF-α and IL-1β levels. However, it potentiated the decrease body weight, colonic SOD activity, GSH and IL-10 levels. Moreover, it ameliorated the marked macroscopic and microscopic changes of colonic mucosa in a dose dependent manner.
Garlic oil inhibits DSS-induced colitis in rats may be through its anti-oxidant, anti-inflammatory and immunomodulatory properties. Therefore, GO could be a promising protective agent recommended for UC patients.
大蒜油（GO）在民间医学中用于治疗多种疾病（包括炎症性疾病）已有数百年历史。最近，它已表现出强大的抗氧化、抗炎和免疫调节作用。因此，我们评估了GO在葡聚糖硫酸钠（DSS）诱导的大鼠结肠炎模型中的可能保护作用。
从第1天到第7天，让大鼠自由饮用含5% DSS的饮用水7天以诱导结肠炎。GO以25、50和100mg/kg/天的剂量口服给药。美沙拉嗪作为标准药物，剂量为15mg/kg/天。所有动物在给药前1小时和给药后1小时禁食2小时，给药从第1天到第7天，每天上午10:00至11:00进行，持续7天。评估动物体重、结肠重量、结肠髓过氧化物酶（MPO）和超氧化物歧化酶（SOD）活性、结肠还原型谷胱甘肽（GSH）、丙二醛（MDA）、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和IL-10水平，以及结肠组织的宏观和微观变化。
GO治疗显著抑制了结肠重量、MPO活性、MDA、TNF-α和IL-1β水平的升高。然而，它加剧了体重下降、结肠SOD活性、GSH和IL-10水平的降低。此外，它以剂量依赖的方式改善了结肠黏膜明显的宏观和微观变化。
大蒜油抑制大鼠DSS诱导的结肠炎可能是通过其抗氧化、抗炎和免疫调节特性。因此，GO可能是一种有前景的推荐给溃疡性结肠炎（UC）患者的保护剂。
