Fujii H, Miwa I, Okuda J
Biochem Int. 1986 Aug;13(2):359-65.
Lactate output from the alpha and beta anomers of glucose was measured in intact human erythrocytes at 37 degrees C; and glucose anomer phosphorylation, in human erythrocyte homogenates. The rates of both glucose metabolism (lactate output) and phosphorylation were higher in the presence of beta-D-glucose as distinct from alpha-D-glucose at three glucose concentrations used (2, 5, and 10 mM). Thus, the v beta/v alpha ratios of metabolism and phosphorylation of glucose at 2 mM were 1.24 and 1.22, respectively. The results indicate that the beta preference of hexokinase, a rate-limiting enzyme in glycolysis, is reflected in beta-preferential glycolysis.
在37摄氏度下，对完整的人体红细胞中葡萄糖的α和β异头物的乳酸生成量进行了测定；并对人体红细胞匀浆中的葡萄糖异头物磷酸化情况进行了测定。在所使用的三种葡萄糖浓度（2、5和10 mM）下，与α-D-葡萄糖不同，在β-D-葡萄糖存在的情况下，葡萄糖代谢（乳酸生成量）和磷酸化的速率均更高。因此，在2 mM时，葡萄糖代谢和磷酸化的vβ/vα比值分别为1.24和1.22。结果表明，糖酵解中的限速酶己糖激酶对β的偏好反映在β优先的糖酵解中。
