Platonova O V, Agranenko V A, Ataullakhanov F I, Vitvitskiĭ V M, Kiiatkina N V
Biokhimiia. 1986 Aug;51(8):1384-91.
The glucose consumption rate versus ATP content in human red cells (regulatory patterns of glycolysis) and ATP concentration versus glucose uptake rate in red cell suspension (regulatory patterns of total ATPases), when the rate of glucose uptake is constant and lower than the rate of glucose consumption at physiological conditions, were measured at different pH values. The shape of both types of kinetic curves was found to be dependent on the pH of the incubation medium but the same for the red cells taken from different donors. It is supposed that at alkaline pH, glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase reactions become the rate-limiting steps of glycolysis instead of hexokinase and phosphofructokinase under physiological conditions.
当葡萄糖摄取速率恒定且低于生理条件下的葡萄糖消耗速率时，在不同pH值下测量了人类红细胞中葡萄糖消耗速率与ATP含量的关系（糖酵解的调节模式）以及红细胞悬液中ATP浓度与葡萄糖摄取速率的关系（总ATP酶的调节模式）。发现这两种类型的动力学曲线形状均取决于孵育介质的pH值，但从不同供体获取的红细胞的曲线形状相同。据推测，在碱性pH条件下，3-磷酸甘油醛脱氢酶和磷酸甘油酸激酶反应成为糖酵解的限速步骤，而不是生理条件下的己糖激酶和磷酸果糖激酶。
