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马铃薯()中基因家族的全基因组鉴定、特征分析、进化及表达分析

Genome-wide identification, characterization, evolution and expression analysis of the gene family in potato ().

作者信息

Jia Wenqi, Xiong Yuting, Li Man, Zhang Shengli, Han Zhongcai, Li Kuihua

机构信息

Agricultural College, Yanbian University, Yanji, Jilin, China.

Jilin Academy of Vegetable and Flower Science, Changchun, Jilin, China.

出版信息

Front Genet. 2023 Aug 23;14:1224015. doi: 10.3389/fgene.2023.1224015. eCollection 2023.

Abstract

The () gene is a key player in environmental stress response and has been identified in many multidimensional tube plant species. However, there are few studies on the gene in potato. In this study, we used genome-wide identification to identify 31 genes in potato. Among the 12 potato chromosomes, the gene was distributed on 11 chromosomes, among which the third chromosome did not have a family member, while the tenth chromosome had the most members with 11 members. 22 of the 31 had a classical gene structure, with one exon and no intron. The conserved DIR domain accounts for most of the proteins in the 27 . The structure of the gene was analyzed and ten different motifs were detected. The gene was divided into three groups according to its phylogenetic relationship, and 22 duplicate genes were identified. In addition, four kinds of cis-acting elements were detected in all 31 promoter regions, most of which were associated with biotic and abiotic stress. The findings demonstrated that the StDIR gene exhibited specific responses to cold stress, salt stress, ABA, and drought stress. This study provides new candidate genes for improving potato's resistance to stress.

摘要

()基因是环境胁迫响应中的关键因子,已在许多多维度管状植物物种中被鉴定出来。然而,关于该基因在马铃薯中的研究却很少。在本研究中,我们通过全基因组鉴定在马铃薯中鉴定出31个()基因。在12条马铃薯染色体中,()基因分布在11条染色体上,其中第三条染色体没有家族成员,而第十条染色体成员最多,有11个。31个()基因中有22个具有经典的()基因结构,有一个外显子且无内含子。保守的DIR结构域占27个()蛋白的大部分。对()基因的结构进行了分析,检测到10种不同的基序。根据系统发育关系,()基因被分为三组,并鉴定出22个重复基因。此外,在所有31个()基因的启动子区域检测到四种顺式作用元件,其中大部分与生物和非生物胁迫相关。研究结果表明,StDIR基因对冷胁迫、盐胁迫、脱落酸和干旱胁迫表现出特异性响应。本研究为提高马铃薯的抗逆性提供了新的候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/626e/10481866/f4bc0099a88f/fgene-14-1224015-g001.jpg

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