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过量表达甘蔗基因可增强. 的耐旱性

Overexpression of Sugarcane Genes Enhances Drought Tolerance in .

机构信息

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China.

Guangxi Key Laboratory of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning 530004, China.

出版信息

Int J Mol Sci. 2022 May 10;23(10):5340. doi: 10.3390/ijms23105340.

DOI:10.3390/ijms23105340
PMID:35628151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9141896/
Abstract

Dirigent proteins (DIRs) are known to function in lignin biogenesis and to be involved in stress resistance in plants. However, the sugarcane DIRs have not been functionally characterized. In this study, we investigated the DIR-protein-encoding genes in spp. (ScDIR) by screening collections of sugarcane databases, monitoring the responses of these genes to drought stress by real-time quantitative PCR, and identifying their heterologous expression in tobacco. Of the 64 identified, four belonging to the DIR-b/d ( and ) and DIR-c ( and ) subfamilies showed a significant transcriptional response when subjected to drought stress. ScDIR5, ScDIR7, and ScDIR11 are localized in the cell membrane, whereas ScDIR40 is found in the cell wall. The overexpression of these genes in tobacco generally increased the drought tolerance of the transgenic lines, with ScDIR7 conferring the highest degree of drought tolerance. The characterization of the physiological and biochemical indicators (superoxide dismutase, catalase, malondialdehyde, and HO) confirmed that the ScDIR-overexpressing lines outperformed the wild type. These results demonstrated that specific in sugarcane respond and contribute to tolerance of drought stress, shedding light on potential means of improving drought tolerance in this crop.

摘要

dirigentes （DIRs）在木质素生物合成中起作用，并且与植物的抗逆性有关。然而，甘蔗 DIRs 的功能尚未得到充分表征。在这项研究中，我们通过筛选甘蔗数据库的集合，实时定量 PCR 监测这些基因对干旱胁迫的反应，并鉴定它们在烟草中的异源表达，研究了 spp.（ScDIR）中的 DIR 蛋白编码基因。在鉴定的 64 个基因中，有 4 个属于 DIR-b/d（和）和 DIR-c（和）亚家族，在受到干旱胁迫时表现出显著的转录响应。ScDIR5、ScDIR7 和 ScDIR11 定位于细胞膜，而 ScDIR40 则位于细胞壁。这些基因在烟草中的过表达通常会提高转基因系的耐旱性，其中 ScDIR7 赋予最高的耐旱性。对生理生化指标（超氧化物歧化酶、过氧化氢酶、丙二醛和 HO）的特征分析证实，ScDIR 过表达系优于野生型。这些结果表明，甘蔗中特定的 DIRs 会对干旱胁迫做出响应，并有助于提高其耐旱性，为提高该作物的耐旱性提供了潜在手段。

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